Lignocellulosic biomass is considered the raw material of the future to produce ethanol, due to its low cost and its enormous availability. Lignocellulosic enzymes play a crucial role in the costs and sustainability of biorefining processes by promoting the biomass fractionation. In this study, the melon waste was used as inducer source to produce lignocellulolytic enzymes by Aspergillus niger M4 strain under submerged fermentation (SmF). The chemical characterization of the whole fruit showed a high content of sugars, as well as hemicellulose, followed by lignin and cellulose. The dried and crushed melon was used as a substrate in submerged fermentation, to induce the synthesis of lignocellulosic enzymes. The Aspergillus niger M4 strain was able to grow and synthesize lignocellulose degrading enzymes, allowing free-glucose values of 3.7 g/L. The culture broth showed endo-glucanase activity (1.21 U/mL), xylanase activity (11.00 U/mL), and laccase activity (18.23 U/mL). The entire melon (Cucumis melo L.) wastes can be used as a substrate in the lignocellulosic enzyme production by submerged fermentation process for further application in the biomass pretreatment for bioethanol production.

木质纤维素生物质因其低成本和巨大的可用性而被认为是未来生产乙醇的原料。木质纤维素酶通过促进生物质分离，在生物精制过程的成本和可持续性中起着至关重要的作用。在这项研究中，甜瓜废料被用作诱导剂来源，在深层发酵（SmF）下由黑曲霉M4菌株产生木质纤维素分解酶。整个水果的化学特性显示出高含量的糖以及半纤维素，其次是木质素和纤维素。将干燥和压碎的瓜用作深层发酵的底物，以诱导木质纤维素酶的合成。该黑曲霉M4菌株能够生长并合成木质纤维素降解酶，游离葡萄糖值为3.7 g / L。培养液显示出内切葡聚糖酶活性（1.21 U / mL），木聚糖酶活性（11.00 U / mL）和漆酶活性（18.23 U / mL）。整个瓜（Cucumis melo L.）废物可通过浸没式发酵过程用作木质纤维素酶生产中的底物，以进一步应用于生产生物乙醇的生物质预处理中。