A newly identified cellulase-producing Fusarium chlamydosporum HML278 was cultivated under solid-state fermentation of sugarcane bagasse, and two new β-glucosides enzymes (BG FH1, BG FH2) were recovered from fermentation solution by modified non-denaturing active gel electrophoresis and gel filtration chromatography. SDS-PAGE analysis showed that the molecular weight of BG FH1 and BG FH2 was 93 kDa and 52 kDa, respectively, and the enzyme activity was 5.6 U/mg and 11.5 U/mg, respectively. The optimal reaction temperature of the enzymes was 60 ℃, and the enzymes were stable with a temperature lower than 70 ℃. The optimal pH of the purified enzymes was 6.0, and the enzymes were stable between pH 4–10. K_m and V_max values ​​were 2.76 mg/mL and 20.6 U/mg for pNPG, respectively. Thin-layer chromatography and high-performance liquid chromatography analysis showed that BG FH1and BG FH2 had hydrolysis activity toward cellobiose and could hydrolyze cellobiose into glucose. In addition, both enzymes exhibited transglycoside activity, which could use glucose to synthesize cellobiose and cellotriose, and preferentially synthesize alcohol. In conclusion, our study demonstrated that F. chlamydosporum HML278 produces heat-resistant β-glucosidases with both hydrolytic activity and transglycosidic activity, and these β-glucosidases have potential application in bioethanol and papermaking industries.

在甘蔗渣的固态发酵中培养了新鉴定的产纤维素酶的衣原体镰刀菌HML278，并通过改良的非变性活性凝胶电泳和凝胶过滤从发酵液中回收了两种新的β-葡萄糖苷酶（BG FH1，BG FH2）。层析。SDS-PAGE分析表明，BG FH1和BG FH2的分子量分别为93 kDa和52 kDa，酶活性分别为5.6 U / mg和11.5 U / mg。酶的最佳反应温度为60℃，在低于70℃的温度下稳定。纯化后的酶的最适pH为6.0，酶在pH 4-10之间稳定。K _m和V _maxpNPG的值分别为2.76 mg / mL和20.6 U / mg。薄层色谱和高效液相色谱分析表明，BG FH1和BG FH2对纤维二糖具有水解活性，可以将纤维二糖水解为葡萄糖。此外，这两种酶均具有转糖苷活性，可以利用葡萄糖合成纤维二糖和纤维三糖，并优先合成醇。总之，我们的研究表明，衣原体霍乱弧菌HML278产生具有水解活性和转糖苷活性的耐热性β-葡萄糖苷酶，这些β-葡萄糖苷酶在生物乙醇和造纸工业中具有潜在的应用前景。