Layer-by-layer (LbL) self-assembly is one of the unique methods for fabrication of ultra-thin film. This technique has immense application aross many fields in today’s life. In this paper, we have presented the fabrication of the thin film comprising of antifungal and antibacterial cationic dye methylene blue with negatively charged E. coli K30 bacterial polysaccharide sharing with PAH. The multilayers were characterized by UV–Vis absorption spectra and AFM. Various photophysical behaviors were investigated. Such studies provide a new light in the research of layer by layer self-assembly technique.

中文翻译：

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使用逐层自组装技术的亚甲基蓝在大肠杆菌K30胶囊多糖上的动态行为

逐层（LbL）自组装是制造超薄膜的独特方法之一。这项技术在当今生活的许多领域都有巨大的应用。在本文中，我们介绍了由抗真菌和抗菌阳离子染料亚甲基蓝与带负电的大肠杆菌 K30 细菌多糖与 PAH 共享薄膜的制备。通过紫外-可见吸收光谱和原子力显微镜对多层膜进行表征。研究了各种光物理行为。这些研究为逐层自组装技术的研究提供了新的思路。