Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2 . Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.

中文翻译：

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Prime 编辑在玉米的两个 ALS 基因中有效产生 W542L 和 S621I 双突变


Prime编辑是最近开发的一种新型、通用的CRISPR/Cas衍生的精准基因组编辑技术。然而，在转基因水稻品系中，引物编辑的效率较低。我们假设增强 pegRNA 表达可以提高引物编辑效率。在本报告中，我们描述了增强 pegRNA 表达的两种策略。我们构建了一个 Prime 编辑载体，其中包含 ZmALS1 和 ZmALS2 中 W542L 和 S621I 双突变的两个 pegRNA 变体。与之前的水稻报道相比，我们在玉米中实现了更高的引物编辑效率。我们的结果令人鼓舞，并为植物主要编辑器的优化提供了方向。