Previous studies have shown that seizures can cause cognitive disorders. On the other hand, the Curcuma zedoaria (CZ) has beneficial effects on the nervous system. However, there is little information on the possible effects of the CZ extract on seizures. The aim of this study was to investigate the possible effects of CZ extract on cognitive impairment and oxidative stress induced by epilepsy in rats. Rats were randomly divided into different groups. In all rats (except the sham group), kindling was performed by intraperitoneal injection of pentylenetetrazol (PTZ) at a dose of 35 mg/kg every 48 h for 14 days. Positive group received 2 mg/kg diazepam + PTZ; treatment groups received 100, 200 or 400 mg/kg CZ extract + PTZ; and one group received 0.5 mg/kg flumazenil and CZ extract + PTZ. Shuttle box and Morris Water Maze tests were used to measure memory and learning. On the last day of treatments PTZ injection was at dose of 60 mg/kg, tonic seizure threshold and mortality rate were recorded in each group. After deep anesthesia, blood was drawn from the rats’ hearts and the hippocampus of all rats was removed. Statistical analysis of the data showed that the CZ extract significantly increased the tonic seizure threshold and reduced the pentylenetetrazol-induced mortality and the extract dose of 400 mg/kg was selected as the most effective dose compared to the other doses. It was also found that flumazenil (a GABAA receptor antagonist) reduced the tonic seizure threshold compared to the effective dose of the extract. The results of shuttle box and Morris water maze behavioral tests showed that memory and learning decreased in the negative control group and the CZ extract treatment improved memory and learning in rats. The CZ extract also increased antioxidant capacity, decreased MDA and NO in the brain and serum of pre-treated groups in compared to the negative control group. Conclusion: It is concluded that the CZ extract has beneficial effects on learning and memory impairment in PTZ-induced epilepsy model, which has been associated with antioxidant effects in the brain or possibly exerts its effects through the GABAergic system.

中文翻译：

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莪术水醇提取物对戊四唑致大鼠癫痫发作后学习记忆障碍及脑组织氧化损伤的影响

先前的研究表明，癫痫发作会导致认知障碍。另一方面，姜黄 (CZ) 对神经系统有益。然而，关于 CZ 提取物对癫痫发作可能产生的影响的信息很少。本研究的目的是研究 CZ 提取物对癫痫引起的大鼠认知障碍和氧化应激的可能影响。将大鼠随机分成不同的组。在所有大鼠（假手术组除外）中，通过腹腔注射戊四唑（PTZ）以每48小时35mg / kg的剂量进行点燃，持续14天。阳性组接受2mg/kg地西泮+PTZ；治疗组接受 100、200 或 400 mg/kg CZ 提取物 + PTZ；一组接受 0.5 mg/kg 氟马西尼和 CZ 提取物 + PTZ。Shuttle box 和 Morris Water Maze 测试用于测量记忆和学习。在治疗的最后一天以60mg/kg的剂量注射PTZ，记录每组的强直发作阈值和死亡率。深度麻醉后，从大鼠心脏抽血，取出所有大鼠的海马体。数据统计分析表明，CZ 提取物显着增加了强直性癫痫阈值并降低了戊四唑诱导的死亡率，与其他剂量相比，400 mg/kg 的提取物剂量被选为最有效剂量。还发现，与提取物的有效剂量相比，氟马西尼（一种 GABAA 受体拮抗剂）降低了强直发作阈值。穿梭箱和Morris水迷宫行为测试结果表明，阴性对照组的记忆和学习能力下降，而CZ提取物治疗改善了大鼠的记忆和学习能力。与阴性对照组相比，CZ 提取物还增加了抗氧化能力，降低了大脑和血清中的 MDA 和 NO。结论：得出的结论是，CZ 提取物对 PTZ 诱导的癫痫模型中的学习和记忆障碍具有有益作用，这与大脑中的抗氧化作用有关或可能通过 GABA 能系统发挥作用。与阴性对照组相比，预处理组脑和血清中的 MDA 和 NO 降低。结论：得出的结论是，CZ 提取物对 PTZ 诱导的癫痫模型中的学习和记忆障碍具有有益作用，这与大脑中的抗氧化作用有关或可能通过 GABA 能系统发挥作用。与阴性对照组相比，预处理组脑和血清中的 MDA 和 NO 降低。结论：得出的结论是，CZ 提取物对 PTZ 诱导的癫痫模型中的学习和记忆障碍具有有益作用，这与大脑中的抗氧化作用有关或可能通过 GABA 能系统发挥作用。