Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

用于检测口蹄疫病毒（FMDV）的非结构蛋白（NSP）抗体的血清学检测方法在流行国家用于疾病监测，对于提供有或没有疫苗接种的疾病自由证据以及恢复状况至关重要爆发后一个国家的自由状态。在一项5地点实验室间的研究中，我们比较了2种商用NSP ELISA试剂盒（ID Screen FMD NSP ELISA单日[短期]和过夜方案，ID.Vet； PrioCHECK FMDV NS抗体ELISA，Thermo Fisher Scientific）的性能。对于过夜和短暂的ID Screen孵育方案，PrioCHECK和ID Screen测试之间的总体一致性分别为93.8％（95％CI：92.0–95.2％）和94.8％（95％CI：93.1–96.1％）。