A cellular stimulation device with a pressurized chamber is developed to investigate the effect of ultrasound and pressure fluctuation on nucleus pulposus (NP) cells. The pressurized chamber is designed to emulate the in vivo environment of intervertebral discs, which are under dynamic pressure, and to emulate impact during sports and exercise. Both hydrostatic pressure and ultrasound stimulation increase phosphorylation of ERK (pERK) in NP cells, and promote its translocation into nucleus. This increase in pERK levels might be activated through calcium signaling pathways as intracellular calcium in NP cells was strongly elevated by pressure changes.

中文翻译：

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髓核细胞对压力和超声刺激的反应

开发了带有加压腔的细胞刺激装置，以研究超声和压力波动对髓核（NP）细胞的影响。加压室设计为模拟椎间盘在动态压力下的体内环境，并模拟运动和锻炼过程中的撞击。静水压力和超声刺激均会增加NP细胞中ERK（pERK）的磷酸化，并促进其向核内转运。pERK水平的这种升高可能通过钙信号传导途径激活，因为NP细胞中的细胞内钙通过压力变化而强烈升高。