Abstract The purpose of this research was to determine mitochondrial and lysosomal damage and oxidative stress status in blood lymphocytes of lead-zinc miners. This research was performed in 10 mine workers who have been in contact with lead and zinc in comparison to a control group containing 10 healthy volunteers. Lymphocytes were isolated from peripheral blood using the Ficoll standard method and then mitochondrial and lysosomal damage and oxidative stress were evaluated. The level of reactive oxygen species (ROS), collapse in the mitochondrial membrane potential (MMP) collapse, and glutathione disulfide (GSSG) content, and lysosomal damage in miners were higher than the control group. Also, viability and glutathione (GSH) content were decreased. The lymphocytes of workers of a lead-zinc mine are more susceptible to oxidative stress, mitochondrial and lysosomal damage. The proper use of safety equipment can reduce the risk of toxic agents and their subsequent hazards for mine workers.

中文翻译：

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铅锌矿的职业暴露诱导矿工淋巴细胞氧化应激：线粒体/溶酶体损伤的作用

摘要 本研究的目的是确定铅锌矿工血液淋巴细胞的线粒体和溶酶体损伤以及氧化应激状态。这项研究是在 10 名接触过铅和锌的矿工中进行的，对照组包含 10 名健康志愿者。使用 Ficoll 标准方法从外周血中分离淋巴细胞，然后评估线粒体和溶酶体损伤和氧化应激。矿工活性氧（ROS）水平、线粒体膜电位崩溃（MMP）崩溃、谷胱甘肽二硫化物（GSSG）含量和溶酶体损伤均高于对照组。此外，活力和谷胱甘肽 (GSH) 含量降低。铅锌矿工人的淋巴细胞更容易受到氧化应激，线粒体和溶酶体损伤。正确使用安全设备可以降低有毒物质的风险及其对矿工的后续危害。