Chitosan coating plus silicon dioxide nanoparticles and nisin were applied on fresh blueberry samples in order to find out safety packaging assay during the post-harvest process. Studies were performed in-vitro for fruit quality as physicochemical parameters and oxidation, while microbiological analyses as molds/yeast and mesophilics populations were examined in-vivo. The selected silicon dioxide nanoparticles 1% and nisin 1%, were added into a chitosan solution, which resulted in four groups of coated blueberries. After storage at ambient temperature, fruits were examined for two, four, six, and eight days. It was noticed that the hardness, chewiness, and cohesiveness of all blueberry samples were increased during the storage. Chitosan-nano-silicon dioxide (CHN-Nano) and (CHN-N-Nano) with the addition of nisin helped to control shrinking (38.52%) and decay rates (8.61%). Moreover, (CHN-N-Nano) reported the lowest L* values (10.54) for the color index, and inhibited the microbial populations (3.60 and 2.73 log CFU/g) for molds/yeast and mesophilics, respectively. (CHN-Nano) reported the lowest value for ph (2.61) and the highest for anthocyanin content (75.19 cyanidin-3-glucoside mg/100 g). The chitosan coating substantially maintained Vitamin C (7.34 mg/100 g) and polyphenoloxidase (PPO) (558.03 U min⁻¹·g⁻¹). The results suggest that nano-material with chitosan film coatings that contained nisin were effective for fresh blueberry preservation under ambient temperature.

中文翻译：

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壳聚糖，乳链菌肽，二氧化硅纳米颗粒涂膜对蓝莓（越桔越橘）品质的影响

为了在收获后的过程中进行安全包装测定，将壳聚糖涂层，二氧化硅纳米颗粒和乳酸链球菌素涂在新鲜的蓝莓样品上。在体外进行了水果质量的理化参数和氧化研究，并在体内检查了作为霉菌/酵母菌和嗜温菌种群的微生物分析。将选定的1％的二氧化硅纳米颗粒和1％的乳链菌肽添加到壳聚糖溶液中，得到四组带涂层蓝莓。在室温下储存后，检查水果两，四，六和八天。注意到在储存过程中所有蓝莓样品的硬度，耐嚼性和内聚性均增加。壳聚糖-纳米二氧化硅（CHN-Nano）和（CHN-N-Nano）以及乳链菌肽的加入有助于控制收缩（38。52％）和衰减率（8.61％）。此外，（CHN-N-Nano）报告的最低L *值（10.54）代表颜色指数，霉菌/酵母菌和嗜温菌分别抑制微生物种群（3.60和2.73 log CFU / g）。（CHN-Nano）的ph值最低（2.61），花青素含量最高（75.19 cyanidin-3-glucoside mg / 100 g）。壳聚糖涂层基本上保持了维生素C（7.34 mg / 100 g）和多酚氧化酶（PPO）（558.03 U min ^-1 ·g ^-1）。结果表明，含有乳链菌肽的壳聚糖薄膜包衣的纳米材料在环境温度下对新鲜蓝莓的保存有效。