Abstract

The present study focused on the combination of biphasic calcium phosphate (BCP) nanoparticles into the modified hyaluronic acid based injectable hydrogels for bone tissue engineering. Self-cross-linked thiolated hyaluronic acid (HA-HS) injectable hydrogels loaded with biphasic calcium phosphate (BCP) nanoparticles were prepared by disulfide cross-linking to mimic the extracellular matrix as a potential material for bone treatment. Varying concentration of HA-HS ranging between 1 and 5w/v% was tested to optimize the optimum concentration and were further modified with varying BCP concentrations for final optimization. Physico-chemical characterizations of the prepared hydrogel such as SEM, EDS, FT-IR, and XRD confirmed that the BCP has effectively loaded and distributed homogeneously in the HA-HS hydrogel. The results showed that the 3% (w/v) HA-HS hydrogel exhibits the appropriate properties for injectable hydrogel system such as gelation times, swelling rate and in vitro degradation behavior among all tested concentrations. Cell viability and cell proliferation using osteoblast cells (MC3T3-E1) demonstrated that the BCP laden modified hydrogel are biocompatible in vitro. In light of the encouraging results obtained, BCP laden HA-HS hydrogels might offer the potential to be used as injectable hydrogel in bone tissue engineering.

摘要

本研究的重点是将双相磷酸钙 (BCP) 纳米颗粒结合到用于骨组织工程的改性透明质酸基可注射水凝胶中。通过二硫化物交联模拟细胞外基质作为骨治疗的潜在材料，制备了负载双相磷酸钙 (BCP) 纳米颗粒的自交联硫醇化透明质酸 (HA-HS) 可注射水凝胶。测试了 1 到 5w/v% 之间的不同浓度的 HA-HS 以优化最佳浓度，并通过不同的 BCP 浓度进一步修改以进行最终优化。所制备的水凝胶的物理化学表征，如 SEM、EDS、FT-IR 和 XRD，证实 BCP 已有效地加载并均匀分布在 HA-HS 水凝胶中。所有测试浓度之间的体外降解行为。使用成骨细胞 (MC3T3-E1) 的细胞活力和细胞增殖表明，负载 BCP 的修饰水凝胶在体外具有生物相容性。鉴于所获得的令人鼓舞的结果，载有 BCP 的 HA-HS 水凝胶可能提供在骨组织工程中用作可注射水凝胶的潜力。