ABSTRACT

Although chemotherapy is one of the effective means of treating gastric cancer, the resistance of chemotherapeutic drugs has followed. And the mechanisms of resistance are not completely clear. The main aim of this article was to develop a kind of drug that could reduce the resistance of cisplatin on gastric cancer cells.

The MGC-803 and MGC-803/DDP cells were treated by cisplatin for 48 h and Lidocaine (Lido) for 24 h. Cell viability, apoptosis, migration and invasion were tested by cell counting kit-8 (CCK-8) assay, apoptosis assay, western blot, migration and invasion assay. After MGC-803/DDP cells were transfected for 48 h, the expression of microRNA-10b (miR-10b) were detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Activation of AKT/mTOR and β-catenin pathways was tested by western blot.

Cisplatin caused MGC-803 and MGC-803/DDP cell apoptosis, and MGC-803/DDP cells possessed higher cisplatin resistance than MGC-803 cells. Lido reduced the cisplatin resistance of MGC-803/DDP cells. Besides, Lido inhibited MGC-803/DDP cell migration and invasion. In addition, Lido declined cisplatin resistance by down-regulating miR-10b. Lido also repressed AKT/mTOR and β-catenin pathway by down-regulating miR-10b.

This article explained the role of Lido in cisplatin resistance in MGC-803/DDP cells. Furthermore, Lido weakened the cisplatin resistance in MGC-803/DDP cells at least in part through decreasing the expression of miR-10b.

摘要

尽管化学疗法是治疗胃癌的有效手段之一，但其对化学治疗药物的抵抗力却随之而来。抗药性的机制尚不完全清楚。本文的主要目的是开发一种可降低顺铂对胃癌细胞的耐药性的药物。

用顺铂处理MGC-803和MGC-803 / DDP细胞48小时，利多卡因（Lido）处理24小时。通过细胞计数试剂盒8（CCK-8）测定，凋亡测定，蛋白质印迹，迁移和侵袭测定来测试细胞活力，凋亡，迁移和侵袭。将MGC-803 / DDP细胞转染48小时后，通过定量逆转录聚合酶链反应（qRT-PCR）检测microRNA-10b（miR-10b）的表达。AKT / mTOR和β-catenin途径的激活通过蛋白质印迹进行了测试。

顺铂引起MGC-803和MGC-803 / DDP细胞凋亡，而MGC-803 / DDP细胞比MGC-803细胞具有更高的顺铂耐药性。Lido降低了MGC-803 / DDP细胞的顺铂耐药性。此外，丽都抑制了MGC-803 / DDP细胞的迁移和侵袭。此外，Lido通过下调miR-10b来降低顺铂耐药性。丽都还通过下调miR-10b抑制AKT / mTOR和β-catenin途径。

本文解释了Lido在MGC-803 / DDP细胞中对顺铂耐药的作用。此外，Lido至少部分地通过降低miR-10b的表达来减弱MGC-803 / DDP细胞的顺铂耐药性。