ABSTRACT

Breast cancer is one of the dominant cancers of women-related death universal. This inquiry aims to disclose the probable role of circABCC4 in breast cancer. The level of circABCC4 was discovered through qRT-PCR. The reactions of circABCC4 and miR-154-5p on the cell viability, apoptosis, migration as well as invasion were, respectively, inspected by CCK-8, flow cytometry, and transwell assays. The association betwixt circABCC4 and miR-154-5p was investigated. The accumulation of NF-κB and Wnt/β-catenin pathway proteins was discovered through Western blot. The expression of circABCC4 was far great in tumor tissues than in normal tissues. Knockdown of circABCC4 could subdue cell viability, migration, invasion, and enhance apoptosis in breast cancer cell lines. CircABCC4 negatively regulated the manifestation of miR-154-5p and shared binding sites with the latter. Suppression of miR-154-5p expression partially conversed the repressive effect of circABCC4 knockdown on breast cancer cell viability, migration, invasion, and NF-κB and Wnt/β-catenin pathways. CircABCC4 knockdown repressed breast cancer cells viability, migration, and invasion by up-regulating miR-154-5p via inhibiting NF-κB and Wnt/β-catenin signal pathways.

摘要

乳腺癌是女性普遍死亡的主要癌症之一。这项调查旨在揭示 circABCC4 在乳腺癌中的可能作用。circABCC4 的水平是通过 qRT-PCR 发现的。circABCC4 和 miR-154-5p 对细胞活力、凋亡、迁移和侵袭的反应分别通过 CCK-8、流式细胞术和 transwell 测定进行检查。研究了 circABCC4 和 miR-154-5p 之间的关联。通过蛋白质印迹发现了 NF-κB 和 Wnt/β-catenin 通路蛋白的积累。circABCC4在肿瘤组织中的表达远高于正常组织。circABCC4的敲低可以抑制乳腺癌细胞系的细胞活力、迁移、侵袭并增强细胞凋亡。CircABCC4 负调控 miR-154-5p 的表现并与后者共享结合位点。miR-154-5p 表达的抑制部分逆转了 circABCC4 敲低对乳腺癌细胞活力、迁移、侵袭以及 NF-κB 和 Wnt/β-catenin 通路的抑制作用。CircABCC4敲低通过抑制NF-κB和Wnt/β-catenin信号通路上调miR-154-5p来抑制乳腺癌细胞的活力、迁移和侵袭。