Abstract

Context

3-Bromopyruvate (3-BP) is a glycolytic inhibitor and a putative caloric restriction mimetic.

Objective

We have examined the effect of low-dose administration of 3-BP to rats and assess the CRM effect by measuring an array of biomarkers of oxidative stress.

Materials and methods

Male Wistar young and old rats were administered with a low-dose 3-BP for four weeks.

Results

A significant increase in ROS was observed in 3-BP-treated rats (both young and old), an increase in erythrocyte PMRS (plasma membrane redox system), FRAP (Ferric reducing ability of plasma), catalase and superoxide dismutase activities were also observed. Treatment with 3-BP also reduced protein carbonyl, advanced oxidation protein products, plasma sialic acid, and advanced glycation end products.

Conclusion

Short-term 3-BP treatment can provide protection against oxidant stress. We suggest that 3-BP triggers a hormetic response subsequent to an increase in ROS leading to the induction of a protective defense mechanism.

中文翻译：

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3-溴丙酮酸提高 ROS 并诱导毒物兴奋作用，从而在年轻和年老大鼠中发挥热量限制模拟作用

摘要

语境

3-Bromopyruvate (3-BP) 是一种糖酵解抑制剂和推定的热量限制模拟物。

客观的

我们检查了低剂量 3-BP 对大鼠的影响，并通过测量一系列氧化应激生物标志物评估 CRM 影响。

材料和方法

雄性 Wistar 年轻和年老大鼠被给予低剂量 3-BP 四个星期。

结果

在 3-BP 处理的大鼠（年轻和年老）中观察到 ROS 显着增加，还观察到红细胞 PMRS（质膜氧化还原系统）、FRAP（血浆铁还原能力）、过氧化氢酶和超氧化物歧化酶活性的增加. 用 3-BP 处理还减少了蛋白质羰基、高级氧化蛋白质产物、血浆唾液酸和高级糖基化终产物。

结论

短期 3-BP 治疗可以提供抗氧化应激保护。我们建议 3-BP 在 ROS 增加导致保护性防御机制的诱导后触发兴奋反应。