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Flow‐based SPPS for protein synthesis: A perspective
Peptide Science ( IF 1.5 ) Pub Date : 2020-10-06 , DOI: 10.1002/pep2.24198
Zachary P. Gates 1 , Nina Hartrampf 2
Affiliation  

Flow‐based approaches to solid phase peptide synthesis (SPPS) have been pursued since the method's early days, with anticipated gains in speed, reaction monitoring, and ease of automation. Here, we discuss how these advantages are being realized by synthesis at elevated temperature, facilitated by a 'preheat/activation' loop. This important modification both accelerates peptide synthesis—providing a wealth of new data from in‐line monitoring—and in conjunction with an optimized protocol, extends the length of peptides routinely accessible by stepwise synthesis. Streamlined synthesis of longer peptides will address a major challenge in chemical protein synthesis: preparation of peptide segments for use in chemical ligation. The context of recent results in flow‐based SPPS is discussed, highlighting remaining challenges and future opportunities.

中文翻译:

基于流程的SPPS用于蛋白质合成:一个视角

自该方法的早期以来,就一直在研究基于流的固相肽合成(SPPS)方法,并有望在速度,反应监控和自动化的简化方面获得收益。在这里,我们讨论如何通过在高温下通过“预热/活化”回路促进合成来实现这些优势。这项重要的改进不仅加快了肽的合成(从在线监测中提供了大量新数据),而且还结合了优化的方案,延长了逐步合成常规可及的肽的长度。更长肽的简化合成将解决化学蛋白质合成中的一个主要挑战:制备用于化学连接的肽段。讨论了基于流的SPPS中的最新结果,
更新日期:2020-12-01
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