The production of rhamnolipid (glycolipid) biosurfactant was achieved under optimized conditions from newly isolated bacteria (Pseudomonas Plecoglossicida BP03) from rice mill effluent. The isolated biosurfactant was structurally characterized using FTIR and NMR spectroscopic studies. The obtained biosurfactant (1·39 gL-1 ) showed a variety of applications including larvicidal and pupicidal activity against malarial vector (Anopheles sunadicus). It also exhibited antimicrobial activity against human pathogens, and possessed potent anti-biofilm activity against S. aureus, B. subtilis and A. hydrophila. The obtained biosurfactant showed a dose dependent inhibition of exopolymeric substance (EPS) and growth curve in S. aureus. Furthermore, cytotoxicity assays revealed that the biosurfactant exhibit a cytotoxic potency against the human fibroblastic sarcoma cells Ht-1080. In-silco analysis was also performed using Schrodinger maestro 9·3 against surface protein (SasG) of S. aureus and the resultant analysis revealed an interactive docking score of -3·4 kcal mol-1 . The obtained result indicates that the synthesized economically viable biosurfactant ensures excellent applications toward various fields.

中文翻译：

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多舌假单胞菌BP03鼠李糖脂的表征及应用

鼠李糖脂（糖脂）生物表面活性剂的生产是在优化条件下从碾米厂废水中新分离的细菌（假单胞菌 Plecoglossicida BP03）中实现的。使用 FTIR 和 NMR 光谱研究对分离的生物表面活性剂进行结构表征。获得的生物表面活性剂（1·39 gL-1）显示出多种应用，包括对疟疾载体（Anopheles sunadicus）的杀幼虫和杀蛹活性。它还表现出对人类病原体的抗菌活性，并对金黄色葡萄球菌、枯草芽孢杆菌和嗜水曲霉具有强大的抗生物膜活性。获得的生物表面活性剂对金黄色葡萄球菌中的外聚物质 (EPS) 和生长曲线显示出剂量依赖性抑制作用。此外，细胞毒性试验表明，生物表面活性剂对人成纤维肉瘤细胞 Ht-1080 具有细胞毒性。还使用 Schrodinger maestro 9·3 对金黄色葡萄球菌的表面蛋白 (SasG) 进行了硅内分析，结果分析显示交互式对接评分为 -3·4 kcal mol-1 。所得结果表明，合成的经济可行的生物表面活性剂确保了在各个领域的优异应用。