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Occurrence of a human‐associated microbial source tracking marker and its relationship with faecal indicator bacteria in an urban estuary
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-11-10 , DOI: 10.1111/lam.13405
Sibel Zeki 1 , Asli Aslan 2 , Selmin Burak 1 , Joan B. Rose 3
Affiliation  

One of the main impacts of urban sprawl in rapidly growing countries has been contamination of coastal environments by waterborne pathogens, posing a critical risk to ecosystem and human health. Microbial source tracking (MST) has been a robust tool to identify the origin of these pathogens globally. This study compared the occurrence of a human-associated Bacteroides marker (BT-α) with faecal indicator bacteria (FIB) in an urban estuary (Golden Horn, Istanbul, Turkey). Faecal coliform (culture method), enterococci (both culture and qPCR method) concentrations and physicochemical variables were compared with the BT-α concentrations in monthly collected samples for a year (n=108). Enterococci concentrations detected by culture and qPCR were positively correlated (r = 0.86, p < 0.01) suggesting that qPCR can be an alternative method for monitoring. BT-α marker was positive for 30% of the samples and positively correlated with enterococci (r = 0.61 and r = 0.64 for culture and qPCR methods respectively, p < 0.01). Rainfall had a moderate positive correlation with all faecal/MST indicators suggesting combined sewer overflows also severely impacted estuarine water quality. The high FIB and BT-α concentrations at upper estuary suggested that faecal pollution mainly originated from the peri-urban settlements around two creeks entering the estuary.

中文翻译:

城市河口人类相关微生物源追踪标记的出现及其与粪便指示菌的关系

在快速发展的国家,城市扩张的主要影响之一是沿海环境被水源性病原体污染,对生态系统和人类健康构成重大风险。微生物源追踪 (MST) 一直是在全球范围内识别这些病原体来源的强大工具。本研究比较了城市河口(金角湾,伊斯坦布尔,土耳其)中人类相关拟杆菌标记物 (BT-α) 与粪便指示菌 (FIB) 的发生情况。将粪便大肠菌群(培养方法)、肠球菌(培养和 qPCR 方法)浓度和理化变量与一年内每月收集的样本中的 BT-α 浓度进行比较(n=108)。通过培养和 qPCR 检测到的肠球菌浓度呈正相关(r = 0.86,p < 0。01) 表明 qPCR 可以作为监测的替代方法。BT-α 标记对 30% 的样本呈阳性,并与肠球菌呈正相关(培养和 qPCR 方法分别为 r = 0.61 和 r = 0.64,p < 0.01)。降雨量与所有粪便/MST 指标呈中度正相关,表明联合下水道溢流也严重影响了河口水质。河口上游的高 FIB 和 BT-α 浓度表明,粪便污染主要来自进入河口的两条小溪周围的城郊聚居区。降雨量与所有粪便/MST 指标呈中度正相关,表明联合下水道溢流也严重影响了河口水质。河口上游的高 FIB 和 BT-α 浓度表明,粪便污染主要来自进入河口的两条小溪周围的城郊聚居区。降雨量与所有粪便/MST 指标呈中度正相关,表明联合下水道溢流也严重影响了河口水质。河口上游的高 FIB 和 BT-α 浓度表明粪便污染主要来自进入河口的两条小溪周围的城郊聚居区。
更新日期:2020-11-10
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