Antimicrobial resistance and virulence genes profiles of Arcobacter butzleri strains isolated from back yard chickens and retail poultry meat in Chile,Letters in Applied Microbiology

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Antimicrobial resistance and virulence genes profiles of Arcobacter butzleri strains isolated from back yard chickens and retail poultry meat in Chile
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-11-10 , DOI: 10.1111/lam.13404
Boris Vidal‐Veuthey ₁ , Ronald Jara ₁ , Karla Santander ₁ , Armin Mella ₁ , Sebastián Ruiz ₁ , Luis Collado _{1,

2}

Affiliation

This research aims to investigate the presence and pathogenic potential of Arcobacter in poultry meat samples purchased in the retail market of Valdivia (South of Chile) as well as in fecal samples from backyard chickens from rural areas around this city. The isolates obtained were identified by molecular methods. Furthermore, putative virulence genes were assessed by PCR and the antimicrobial resistance was tested by phenotypic methods. Arcobacter was present in 41.6% of the samples, with the highest value in retail poultry meat (55.7%) followed by backyard production (28.0%). Arcobacter butzleri was the most prevalent species (75.6%) followed by Arcobacter skirrowii (14.8%) and Arcobacter cryaerophilus (9.6%). An 8.5% of A. butzleri strains from meat were resistant to both ciprofloxacin and tetracycline and 6.1% were resistant to erythromycin, while none was resistant to gentamycin, unlike strains from domestic chickens, which showed no resistance. Furthermore, A. butzleri strains from chicken meat presented a higher prevalence of virulence genes than strains from domestic chickens. In fact, in this last group, some genes (hecA, hecB and irgA) were completely absent. Therefore, this study provides insight on the epidemiology of Arcobacter in Chilean poultry and suggests that under traditional breeding conditions strains are, apparently, less pathogenic and drug-resistant.

中文翻译：

从智利的后院鸡和零售家禽肉中分离出的Arcobacter butzleri 菌株的抗菌素耐药性和毒力基因谱

本研究旨在调查从 Valdivia（智利南部）零售市场购买的禽肉样品以及该城市周边农村地区后院鸡的粪便样品中 Arcobacter 的存在和致病潜力。获得的分离物通过分子方法鉴定。此外，通过 PCR 评估假定的毒力基因，并通过表型方法测试抗菌素耐药性。弧菌存在于 41.6% 的样本中，零售禽肉中含量最高 (55.7%)，其次是庭院生产 (28.0%)。Arcobacter butzleri 是最普遍的物种 (75.6%)，其次是 Arcobacterskirrowii (14.8%) 和 Arcobacter craeerophilus (9.6%)。来自肉的 8.5% 的 A. butzleri 菌株对环丙沙星和四环素都有抗性，6.1% 对红霉素有抗性，而没有一种对庆大霉素有抗药性，不像家鸡的品系没有表现出抗药性。此外，来自鸡肉的 A. butzleri 菌株比来自家鸡的菌株表现出更高的毒力基因流行率。事实上，在最后一组中，一些基因（hecA、hecB 和 irgA）完全缺失。因此，这项研究提供了对智利家禽 Arcobacter 流行病学的深入了解，并表明在传统育种条件下，菌株的致病性和耐药性显然较低。hecB 和 irgA) 完全不存在。因此，这项研究提供了对智利家禽 Arcobacter 流行病学的深入了解，并表明在传统育种条件下，菌株的致病性和耐药性显然较低。hecB 和 irgA) 完全不存在。因此，这项研究提供了对智利家禽 Arcobacter 流行病学的深入了解，并表明在传统育种条件下，菌株的致病性和耐药性显然较低。

更新日期：2020-11-10

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