Effect of mating on mRNA and protein expression of beta nerve growth factor and its receptor, TrKA, in the oviduct of llama (Lama glama),Molecular Reproduction and Development

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Effect of mating on mRNA and protein expression of beta nerve growth factor and its receptor, TrKA, in the oviduct of llama (Lama glama)
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-10-06 , DOI: 10.1002/mrd.23426
Luciana M Sari ₁ , Renato Zampini _{1,

2} , Martin E Argañaraz _{1,

2} , Marcelo H Ratto ₃ , Silvana A Apichela _{1,

4}

Affiliation

Copulation produces different stimuli in the female reproductive tract in camelids, which lead to ovulation. Expression of β‐nerve growth factor (β‐NGF) and its specific receptor, tropomyosin receptor kinase A (TrKA), was studied comparing the oviductal microenvironment of mated and nonmated llamas. β‐NGF and TrKA were expressed in the llama ampulla, isthmus, and utero‐tubal‐junction (UTJ), and they were mainly colocalized in the apical region of the oviductal mucosa. A TrKA immunosignal was also found in muscle cells and blood vessels, with the highest mark in UTJ muscle cells of copulated females. Both β‐NGF and TrKA transcripts were expressed in the three oviductal segments. Relative TrKA abundance did not differ between mated and nonmated females, but relative β‐NGF abundance was higher in the UTJ of copulated females (p < .05). β‐NGF might not be secreted into the oviductal fluid (OF) since the protein was not found in the OF of mated or nonmated females. Therefore, it can be concluded that the llama oviduct expresses the β‐NGF/TrKA system and that an increase in β‐NGF gene expression in the UTJ 24 h after copulation along with an increase in TrKA protein expression may indicate an important role in the gamete transport and fertilization process in llamas.

中文翻译：

交配对美洲驼（Lama glama）输卵管中β神经生长因子及其受体TrKA mRNA和蛋白表达的影响

交配会在骆驼科动物的雌性生殖道中产生不同的刺激，从而导致排卵。β-神经生长因子 (β-NGF) 及其特异性受体原肌球蛋白受体激酶 A (TrKA) 的表达被研究，比较已交配和未交配的美洲驼的输卵管微环境。β-NGF 和 TrKA 在美洲驼壶腹、峡部和输卵管交界处 (UTJ) 中表达，它们主要共定位于输卵管黏膜的顶端区域。在肌肉细胞和血管中也发现了 TrKA 免疫信号，在交配雌性的 UTJ 肌肉细胞中标记最高。这两个β-NGF及受体TrKA成绩单三个输卵管段表达。相对TrKA交配和未交配雌性之间的丰度没有差异，但交配雌性的 UTJ 中β-NGF 的相对丰度更高 ( p < .05)。β-NGF 可能不会分泌到输卵管液 (OF) 中，因为在已交配或未交配的雌性的 OF 中未发现该蛋白质。因此，可以得出结论，美洲驼输卵管表达 β-NGF/TrKA 系统，交配后 24 小时 UTJ中β-NGF基因表达的增加以及 TrKA 蛋白表达的增加可能表明在美洲驼的配子运输和受精过程。

更新日期：2020-11-25

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