Phaeodactylum tricornutum is a lipid‐rich marine diatom that contains a high level of omega‐3 polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA). In an effort to reduce costs for large‐scale cultivation of this microalga, this study first established a New BBM medium (0.3 x strength BBM with only 3% of the initial phosphate level) to replace the traditional F/2 medium. Phaeodactylum tricornutum could grow in extremely low phosphate concentrations (25 µM), without compromising the EPA content. In the presence of sea salts, silicate addition was not necessary for high rate growth, high EPA content, or lipid accumulation in this species. Using urea as the sole nitrogen source tended to increase EPA contents per dry biomass (by 24.7%) while not affecting growth performance. The use of sea salts, rather than just sodium chloride, led to significantly improved biomass yields (20% increase) and EPA contents of total fatty acid (46–52% increase), most likely because it supplied sufficient essential elements such as magnesium. A salinity level of 35 led to significantly higher biomass yields compared with 20, but salinity had no significant influence on EPA content. EPA became the dominant fatty acid with average levels of 51.8% of total fatty acids during the exponential growth phase at 20 ppt in New BBM medium with sea salts.

中文翻译：

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三角果盘藻（Phaeodactylum tricornutum）生产高级ω-3二十碳五烯酸（EPA）的开发

三角藻属（Phaeodylumum tricornutum）是一种富含脂质的海洋硅藻，其中含有高水平的omega-3多不饱和脂肪酸，尤其是二十碳五烯酸（EPA）。为了降低大规模养殖该微藻的成本，本研究首先建立了一种新型BBM培养基（0.3 x强度BBM，仅初始磷水平的3％）来替代传统F / 2培养基。玉米角线虫可能以极低的磷酸盐浓度（25 µM）生长，而不会影响EPA含量。在存在海盐的情况下，对于该物种的高速率生长，高EPA含量或脂质积累，不需要添加硅酸盐。使用尿素作为唯一的氮源往往会增加每干生物质的EPA含量（增加24.7％），同时不影响生长性能。使用海盐而不是仅使用氯化钠，可以显着提高生物量的产量（增加20％）和总脂肪酸中EPA含量（增加46-52％），这很可能是因为它提供了足够的必需元素，例如镁。盐度水平为35导致生物质产量显着高于20，但盐度对EPA含量没有显着影响。EPA成为主要脂肪酸，平均含量为51。