IL-18 variant increases risk of enhanced HBV DNA replication in chronic hepatitis,Immunology Letters

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IL-18 variant increases risk of enhanced HBV DNA replication in chronic hepatitis
Immunology Letters ( IF 3.3 ) Pub Date : 2020-10-07 , DOI: 10.1016/j.imlet.2020.10.002
Walid Ben Selma ₁ , Sana Alibi ₂ , Mohamed Ali Smach ₂ , Afef Saad ₃ , Jalel Boukadida ₄

Affiliation

Background

The outcome ofhepatitis B (HBV) infection is influenced by immune responses and host genetics. Interleukin-18 (IL-18) is a determinant factor in controlling the balance of Th1/Th2 during antiviral response.Weexamine therole of two functional polymorphisms -607A/C and-137A/C inIL-18 gene with risk of chronic HBV infection.

Methods and Results

Genomic DNA isolates were obtained from 200 seropositive cases stratified according to their HBV DNA loads, and 200 blood donorsas a control population. Genotypes of the two polymorphisms were identified by ARMS-PCR method. The -607A allele, the-607AA and -607AC genotypes were associated with increased risk to develop chronic HBV infection (1.98, 5.11 and 3.5-fold risks, respectively). By contrast, the -137C minor allele and CG genotype had protected effects against chronic HBV infection.

We found that -607A allele, -607AA and -607AC genotypes were significantly more frequent in patient’s group with high HBV DNA levels compared to patient group with low HBV DNA level. Additionally, they were associated with increased 1.72, 6.04 and 3.28-fold risk of high HBV DNA replication.

Patients carrying “-607A/-137 C” or “-607A/−137 G” haplotypes presented a high risk to develop chronic HBV infection (OR = 3.27; OR = 4.32, respectively).

Conclusions

Taken together, our data suggest that theIL-18 -607A/C functional polymorphism was associated with susceptibility to enhanced replicative form of HBV DNA in chronic infection.

中文翻译：

IL-18 变异增加慢性肝炎中 HBV DNA 复制增强的风险

背景

乙型肝炎 (HBV) 感染的结果受免疫反应和宿主遗传的影响。白细胞介素18（IL-18）是抗病毒反应过程中控制Th1/Th2平衡的决定因素。我们研究IL-18基因中两个功能多态性-607A/C和-137A/C与慢性HBV感染风险的作用.

方法和结果

基因组 DNA 分离物是从 200 个血清阳性病例中获得的，这些病例根据其 HBV DNA 负荷分层，200 名献血者作为对照人群。两种多态性的基因型通过ARMS-PCR方法进行鉴定。-607A 等位基因、-607AA 和 -607AC 基因型与慢性 HBV 感染风险增加相关（风险分别为 1.98、5.11 和 3.5 倍）。相比之下，-137C 次要等位基因和 CG 基因型对慢性 HBV 感染具有保护作用。

我们发现-607A 等位基因、-607AA 和-607AC 基因型在高HBV DNA 水平的患者组中比低HBV DNA 水平的患者组更常见。此外，它们与高 HBV DNA 复制的风险增加 1.72、6.04 和 3.28 倍相关。

携带“-607A/-137 C”或“-607A/-137 G”单倍型的患者发生慢性HBV感染的风险很高（分别为OR = 3.27；OR = 4.32）。