Drosophila nasuta nasuta and Drosophila nasuta albomicans represent cross fertile members of the immigrans species group of Drosophila with an allopatric mode of distribution exhibiting characteristic novelties. Illumina sequencing technology and de novo transcriptome assembling strategies were used for the current study. The analysis revealed 8% of the transcriptome to be differentially expressed between the ovaries of these two species, of which 9% was related to female reproduction. The majority of the differentially expressed genes were enriched for genetic information processing pathways, biosynthesis, and metabolism-related pathways. SNPs in D. n. albomicans transcriptome was double in number than in D. n. nasuta and only 5% of these SNPs were fixed. Ka/Ks ratios indicated the lineages were under strong purifying selection. The genes which are differentially expressed are evolving at a similar rate as that of genes with conserved expression. Thus, the current findings provide useful insights on the expression dynamics during incipient species divergence of D. n. nasuta and D. n. albomicans since their divergence time of ∼0.5 million years.

果蝇nasuta nasuta和果蝇nasuta银额表示的横肥沃成员伊米物种组的果蝇与分布显示出特征的新奇的异域模式。Illumina测序技术和从头转录组组装策略用于当前研究。分析显示这两个物种的卵巢之间有8％的转录组差异表达，其中9％与雌性生殖有关。大多数差异表达基因丰富了遗传信息处理途径，生物合成和代谢相关途径。D中的SNP 。白化菌转录组的数量是D.中的两倍。nasuta，只有5％的SNP是固定的。Ka / Ks比值表明血统处于强力纯化选择之下。差异表达的基因以与保守表达的基因相似的速率进化。因此，目前的发现为D. n。的初始物种发散期间的表达动力学提供了有用的见解。nasuta和D. 银额自〜0.5亿年的分歧时间。