Purified bioactive components of marine algae have shown great pharmaceutical and biomedical potential, including wound healing activity. However, the activity of Spirulina maxima is the least documented with regard to wound healing potential. In the present study, we investigated the regenerative and wound healing activities of a Spirulina (Arthrospira) maxima based pectin (SmP) using in vitro human dermal fibroblasts (HDFs) and in vivo zebrafish model. SmP treated (12.5–50 μg/mL) HDFs showed increased cell proliferation by 20–40% compared to the untreated HDFs. Moreover, in vitro wound healing results in HDFs demonstrated that SmP decreased the open wound area % in concentration-dependent manner at 12.5 (32%) and 25 μg/mL (12%) compared to the control (44%). Further, zebrafish larvae displayed a greater fin regenerated area in the SmP exposed group at 25 (0.48 mm²) and 50 μg/mL (0.51 mm²), whereas the untreated group had the lowest regenerated area (0.40 mm²) at 3 days post amputation. However, fin regeneration was significantly (P < 0.001) higher only in the SmP treated group at 50 μg/mL. Furthermore, the open skin wound healing % in adult zebrafish was significantly higher (P < 0.05) after topical application (600 μg/fish) of SmP (46%) compared to the control (38%). Upregulation of genes such as tgfβ1, timp2b, mmp9, tnf-α, and il-1β, and chemokines such as cxcl18b, ccl34a.4, and ccl34b.4, in the muscle and kidney tissues of SmP treated fish compared to the respective control group was demonstrated using qRT-PCR. Histological analysis results further supported the rapid epidermal growth and tissue remodeling in SmP treated fish, suggesting that SmP exerts positive effects associated with wound healing. Therefore, SmP can be considered a potential regenerative and wound healing agent.

纯化的海藻生物活性成分已显示出巨大的药物和生物医学潜力，包括伤口愈合活性。但是，就伤口愈合潜力而言，最大螺旋藻的活性最少。在本研究中，我们调查了的再生和创伤愈合的活动螺旋藻（节旋藻）最大值使用基于果胶（SMP）体外人皮肤成纤维细胞（HDF）中的和在VIV ö斑马鱼模型。与未经处理的HDF相比，经SmP处理的（12.5–50μg/ mL）HDF显示出细胞增殖提高了20–40％。而且，体外HDFs的伤口愈合结果表明，与对照（44％）相比，SmP以浓度依赖性方式降低了开放伤口面积％（分别为12.5（32％）和25μg/ mL（12％））。此外，斑马鱼幼虫在暴露于SmP的组中显示出更大的鳍再生面积，分别为25（0.48 mm ²）和50μg/ mL（0.51 mm ²），而未处理组在3天时具有最低的再生面积（0.40 mm ²）。截肢后。但是， 仅在50μg/ mL的SmP处理组中，鳍的再生显着更高（P <0.001）。此外，成年斑马鱼的皮肤开放伤口愈合百分比显着更高（P 与对照组（38％）相比，局部施用（600μg/鱼）SmP（46％）后<0.05）。与相应对照相比，在SmP处理过的鱼的肌肉和肾脏组织中，tgfβ1，timp2b，mmp9，tnf-α和il-1β等基因以及cxcl18b，ccl34a.4和ccl34b.4等趋化因子上调。使用qRT-PCR证实了该组。组织学分析结果进一步支持了用SmP处理的鱼的快速表皮生长和组织重塑，表明SmP发挥了与伤口愈合相关的积极作用。因此，SmP可以被认为是潜在的再生和伤口愈合剂。