The chemical signatures emitted by fungal substrates are key components for mycophagous insects in the search for food source or for suitable oviposition sites. These volatiles are usually emitted by the fruiting bodies and mycelia. The volatiles attract fungivorous insects, like flowers attract pollinators; certain flowers mimic the shape of mushroom fruiting bodies and even produce a typical mushroom odor to exploit on fungus-insect mutualism. There are numerous insects which are mycophagous or eat fungi additionally, but only a few are considered a threat in agriculture. Lycoriella ingenua is one of the most serious pests in mushroom cultivation worldwide. Here we attempt to examine the role of environmental volatiles upon behavioral oviposition preference. In two-choice bioassays, fungus gnats preferred uncolonized compost compared to colonized compost but preferred colonized compost against nothing. However, when colonized compost was paired against distilled water, no significant choice was observed. The comparison of fresh casing material and mycelium colonized casing material resulted in no significant preference. From colonized compost headspace, three antennally active volatiles were isolated by gas chromatography coupled with electroantennography and subsequently identified with gas chromatography coupled mass spectrometry as 1-hepten-3-ol, 3-octanone and 1-octen-3-ol. In behavioral assays the addition of said synthetic volatiles to uncolonized compost separately and in combination to mimic colonized compost resulted in avoidance. We thus partially elucidate the role of fungal volatiles in the habitat seeking behavior of Lycoriella ingenua.

真菌底物散发出的化学特征是食虫性昆虫在寻找食物来源或合适产卵位点的关键成分。这些挥发物通常由子实体和菌丝体释放。挥发物会吸引真菌昆虫，就像花朵会吸引传粉媒介一样。某些花朵模仿蘑菇子实体的形状，甚至产生典型的蘑菇气味，以利用真菌-昆虫的共生关系。有许多昆虫是食虫性的或另外吃真菌的，但只有少数被认为对农业构成威胁。蓝藻是全世界蘑菇种植中最严重的害虫之一。在这里，我们尝试检查环境挥发物在行为产卵偏好上的作用。在二选一的生物测定法中，与定殖的堆肥相比，真菌更喜欢未定殖的堆肥，但对什么都没有的定植的堆肥。然而，当将定殖的堆肥与蒸馏水配对时，没有观察到明显的选择。新鲜肠衣材料和菌丝体定殖的肠衣材料的比较没有明显的偏爱。从定殖的堆肥顶空中，通过气相色谱和电触角造影法分离出三种具有天线活性的挥发物，随后通过气相色谱耦合质谱法鉴定为1-庚烯-3-醇，3-辛酮和1-辛烯-3-醇。在行为测定中，将所述合成挥发物分别地添加到未定殖的堆肥中并组合以模拟定殖的堆肥导致避免。因此，我们部分阐明了真菌挥发物在寻求行为的栖息地中的作用。蓝藻。