A simple potentiostat was constructed as a strategy to enhance solvent production in a mediatorless and oxygen-exposed fermentation inoculated with the aerotolerant strain Clostridium sp. C10. Elevated n-butanol and acetone titers were recorded in all fermentations with either glucose or xylose in the presence of electrodes poised at + 500 mV (+ 814 mV vs SHE) relative to cells plus substrate only controls. Respective butanol titers and volumetric butanol productivities in studies performed with 30 g/L glucose or 30 g/L xylose were 1.67 and 2.27 times and 1.90 and 6.13 times greater in the presence of electrodes compared to controls. Glucose and xylose utilization in the presence of electrodes was 61 and 125% greater than no-electrode controls, respectively. Increasing substrate concentrations to 60 g/L decreased the butanol yields relative to the studies performed at 30 g/L. These data suggest that it may be more efficient to alter reactor reduction potential than increase substrate concentration for solvent output during industrial fermentations, which favors higher yield with few additional inputs.

构建了一种简单的恒电位仪，作为在用耐气菌株梭状芽孢杆菌接种的无介体和氧暴露的发酵中提高溶剂产量的策略。sp。C10。相对于仅含底物的对照，在葡萄糖或木糖存在的所有发酵中，在电极保持在+ 500 mV（相对于SHE为+ 814 mV）保持平衡的情况下，记录了所有葡萄糖或木糖发酵的正丁醇和丙酮滴定度的升高。与对照组相比，在有电极的情况下，用30 g / L葡萄糖或30 g / L木糖进行的研究中，丁醇滴度和体积丁醇生产率分别为1.67和2.27倍和1.90和6.13倍。在有电极的情况下，葡萄糖和木糖的利用率分别比无电极的对照组高61和125％。与以30 g / L进行的研究相比，将底物浓度提高至60 g / L会降低丁醇的产量。