Prosthetic vascular graft infection is one of the most severe vascular surgery complications. Fibrin gel (FG) has many useful characteristics as biocompatibility, biodegradation, adhesion, and haemostasis to develop the local antibiotic delivery system. In this study, human plasma was collected from peripheral blood that was used to create fibrin gel by supplement ion Ca 2+ . Antibiotic-containing fibrin gel was then evaluated in some characteristics such as surface structure, biodegradation, antibiotic delivery, cytotoxicity, and bacterial biofilm prevention in vitro and in vivo . The results showed that fibrin gel was excellent material for the extended delivery of antibiotics. Most importantly, antibiotic-containing fibrin gel was not toxic for human fibroblast cells in vitro and inhibited bacterial biofilm growth in vitro and in vivo . This research is the first step in developing an antibiotic delivery system for effective graft infection treatments.

中文翻译：

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开发纤维蛋白凝胶局部万古霉素递送系统预防金黄色葡萄球菌生物膜移植物感染

人工血管移植感染是最严重的血管手术并发症之一。纤维蛋白凝胶 (FG) 具有许多有用的特性，如生物相容性、生物降解、粘附和止血，可用于开发局部抗生素递送系统。在这项研究中，人血浆是从外周血中收集的，用于通过补充离子 Ca 2+ 来制造纤维蛋白凝胶。然后评估了含有抗生素的纤维蛋白凝胶的一些特性，如表面结构、生物降解、抗生素递送、细胞毒性和体外和体内细菌生物膜预防。结果表明，纤维蛋白凝胶是延长抗生素递送的优良材料。最重要的是，含抗生素的纤维蛋白凝胶在体外对人成纤维细胞没有毒性，并在体外和体内抑制细菌生物膜的生长。