This study evaluated the effectiveness of sequential anaerobic/aerobic biodegradation of tetrachloroethene (PCE) and its intermediates, cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC). Two sand columns were operated in series. The first column simulated the up-gradient side of a groundwater system, was operated under anaerobic conditions, and was continuously fed the target contaminant, PCE (42 µM). The second column simulated the down-gradient side of the groundwater system and was operated under aerobic conditions, using low concentrations of hydrogen peroxide as the dissolved oxygen source. After 15 days of operation, cDCE was detected at the end of the first, anaerobic column, at concentrations of 7.02–15.57 μM. After 36 days of operation, VC (7.32 μM) was also detected at the end of the first column. cDCE and VC then migrated into the second, aerobic column. Results showed that cDCE and VC were almost completely aerobically biodegraded in the second column, with removal efficiencies of up to 97% and 95%, respectively. This study also used batch experiments to compare cDCE removal efficiencies between aerobic metabolism using cDCE as the only substrate, and aerobic cometabolism using methane and cDCE as primary and secondary substrates. Results showed that aerobic cometabolism of cDCE was inhibited at cDCE concentrations greater than 50 mg/L. This inhibition effect was not obvious under aerobic metabolism using cDCE as the only substrate. Results of a Michaelis–Menten/Monod kinetics analysis showed that when cDCE concentrations were greater than 20 mg/L, cDCE could be biodegraded more effectively under aerobic metabolism than under aerobic cometabolism.

这项研究评估了四氯乙烯（PCE）及其中间体顺式厌氧/好氧生物降解的顺序有效性-1,2-二氯乙烯（cDCE）和氯乙烯（VC）。两个砂柱串联运行。第一列模拟了地下水系统的上坡侧，在厌氧条件下运行，并连续注入目标污染物PCE（42 µM）。第二列模拟地下水系统的下降梯度侧，并在有氧条件下运行，使用低浓度的过氧化氢作为溶解氧源。手术15天后，在第一个厌氧色谱柱末端检测到cDCE，浓度为7.02–15.57μM。运转36天后，在第一列末端也检测到VC（7.32μM）。然后，将cDCE和VC迁移到第二个有氧色谱柱中。结果表明，cDCE和VC在第二列中几乎完全被好氧生物降解，去除效率分别高达97％和95％。这项研究还使用批处理实验比较了使用cDCE作为唯一底物的有氧代谢与使用甲烷和cDCE作为主要和次要底物的有氧代谢代谢之间的cDCE去除效率。结果表明，当cDCE浓度大于50 mg / L时，可抑制cDCE的有氧代谢。在以cDCE为唯一底物的有氧代谢下，这种抑制作用并不明显。Michaelis-Menten / Monod动力学分析的结果表明，当cDCE浓度大于20 mg / L时，有氧代谢下的cDCE比有氧代谢代谢下的生物降解更有效。这项研究还使用批处理实验比较了使用cDCE作为唯一底物的有氧代谢与使用甲烷和cDCE作为主要和次要底物的有氧代谢代谢之间的cDCE去除效率。结果表明，当cDCE浓度大于50 mg / L时，可抑制cDCE的有氧代谢。在以cDCE为唯一底物的有氧代谢下，这种抑制作用并不明显。Michaelis-Menten / Monod动力学分析的结果表明，当cDCE浓度大于20 mg / L时，有氧代谢下的cDCE比有氧代谢代谢下的生物降解更有效。这项研究还使用批处理实验比较了使用cDCE作为唯一底物的有氧代谢与使用甲烷和cDCE作为主要和次要底物的有氧代谢代谢之间的cDCE去除效率。结果表明，当cDCE浓度大于50 mg / L时，可抑制cDCE的有氧代谢。在以cDCE为唯一底物的有氧代谢下，这种抑制作用并不明显。Michaelis-Menten / Monod动力学分析的结果表明，当cDCE浓度大于20 mg / L时，有氧代谢下的cDCE比有氧代谢代谢下的生物降解更有效。结果表明，当cDCE浓度大于50 mg / L时，可抑制cDCE的有氧代谢。在以cDCE为唯一底物的有氧代谢下，这种抑制作用并不明显。Michaelis-Menten / Monod动力学分析的结果表明，当cDCE浓度大于20 mg / L时，有氧代谢下的cDCE比有氧代谢代谢下的生物降解更有效。结果表明，当cDCE浓度大于50 mg / L时，可抑制cDCE的有氧代谢。在以cDCE为唯一底物的有氧代谢下，这种抑制作用并不明显。Michaelis-Menten / Monod动力学分析的结果表明，当cDCE浓度大于20 mg / L时，有氧代谢下的cDCE比有氧代谢代谢下的生物降解更有效。