Late embryogenesis abundant (LEA) proteins protect organisms from various environmental stresses; however, the underlying mechanism of LEA mediated therapeutic evasion is still unclear in both eukaryotes and prokaryotes. In this study, group 3 LEA protein (G3LEA) of vancomycin-resistant Enterococcus faecium under sublethal concentration of vancomycin stress was evaluated and shown to have two functions: the first is the reduction of reactive oxygen species (ROS) content, preventing apoptosis by suppressing apoptotic proteins Cas3 and MAOB, and the second is activating specific drug efflux pumps. Sublethal vancomycin model was established with using Propidium Iodide (PI) stain. Real-time PCR was conducted to evaluate the expression of G3lea. Flow cytometry and confocal microscope using Anti- G3LEA, anti- MAOB, and anti- Cas3 were performed to assess the expression of G3LEA. Under sublethal vancomycin stress, G3LEA is upregulated, suppressing the expression of apoptotic markers and increasing specific efflux markers. These results suggest that G3LEA protein suppresses antibiotic mediated apoptosis in prokaryotic cells and plays a key role in understanding and preventing antibiotic resistance.

晚期胚胎发育丰富 (LEA) 蛋白保护生物体免受各种环境压力；然而，LEA 介导的治疗逃避的潜在机制在真核生物和原核生物中仍不清楚。本研究评估了亚致死浓度万古霉素胁迫下耐万古霉素屎肠球菌的第 3 组 LEA 蛋白 (G3LEA)并显示其具有两个功能：第一个是减少活性氧 (ROS) 含量，通过抑制细胞凋亡来防止细胞凋亡。凋亡蛋白 Cas3 和 MAOB，第二个是激活特定的药物外排泵。使用碘化丙啶（PI）染色建立亚致死万古霉素模型。进行实时 PCR 以评估G3lea的表达. 使用抗-G3LEA、抗-MAOB和抗-Cas3进行流式细胞术和共聚焦显微镜以评估G3LEA的表达。在亚致死万古霉素应激下，G3LEA 上调，抑制凋亡标志物的表达并增加特异性外排标志物。这些结果表明 G3LEA 蛋白抑制原核细胞中抗生素介导的细胞凋亡，并在理解和预防抗生素耐药性方面发挥关键作用。