Environmental pollutants with endocrine-disrupting effect are of global importance due to their contribution to the aethiologies of variety of complex diseases. These lipophilic pollutants are persistent in the environment and able to bioaccummulate in nontarget organisms. BPA, DEHP and PCB118 (dioxin-like PCB) are associated with endocrine disruption effects, while information on their effects on aquatic invertebrates are limited. In the current study, the effects of these compounds, which are ubiqutous and present at low concentrations in the environment, are studied in the primary hepatopancreas, muscle, gill, intestine and gonadal cultures of narrow-clawed crayfish (Astacus leptodactylus Eschscholtz, 1823), a widely distributed freshwater crayfish in Turkey with high economic importance. IC50 values following MTT assay ranged 0.27–12.61 nM; when compared with other tissues, the gonads were more affected with lower IC50 values. PCB118 induced higher cytotoxicity, while DEHP was the least toxic compound. This is the first study on the primary culture of A. leptodactylus¸ and the toxic effects of these compounds in this organism providing mechanistic insights on the responses and detoxification capacity of the organs. This study provides basis to unravel the mechanism of action of the tested EDCs in crayfish and improvement of cell culture conditions for ecotoxicity and screening assays.

具有内分泌干扰作用的环境污染物具有全球重要性，因为它们对各种复杂疾病的病因学做出了贡献。这些亲脂性污染物在环境中具有持久性，并且能够在非目标生物中进行生物积累。BPA、DEHP 和 PCB118（类二恶英 PCB）与内分泌干扰效应有关，而关于它们对水生无脊椎动物的影响的信息有限。在目前的研究中，这些化合物在环境中普遍存在且以低浓度存在的影响，在窄爪小龙虾（Astacus leptodactylus）的初级肝胰腺、肌肉、鳃、肠和性腺培养物中进行了研究。Eschscholtz, 1823)，一种在土耳其广泛分布的淡水小龙虾，具有很高的经济重要性。MTT 测定后的 IC50 值范围为 0.27–12.61 nM；与其他组织相比，性腺受到的影响更大，IC50 值较低。PCB118 诱导更高的细胞毒性，而 DEHP 是毒性最小的化合物。这是对钩爪蟾的原代培养和这些化合物在该生物体中的毒性作用的首次研究，提供了有关器官反应和解毒能力的机制见解。该研究为揭示受试EDCs在小龙虾中的作用机制以及改善细胞培养条件以进行生态毒性和筛选试验提供了基础。