Sickle cell disease is a group of diseases inherited through the gene and it affects the haemoglobin in the red blood cell. This study investigated the methanol seed extract of Buchholzia coriacea for possible in vitro anti-sickling effects and also determined the effect of Mucuna pruriens seed extract on the solubility and oxygen-binding rate of sickle cell haemoglobin. Sickle cell blood was collected from sickle cell disease patients with subsequent addition of 2% sodium metabisulphite to cause more sickling. Varying concentrations of the seed extracts (50%, 25%, 12.5% and 6.25%) were added to the pre-treated blood for these in vitro assays. The results showed that the extract of Buchholzia coriacea significantly (P < 0.05) inhibited sickling at all concentrations with the highest percentage inhibition of 73.3 ± 5.8, reversed sickled erythrocytes at all concentrations with the highest percentage reversal of 83.3 ± 5.8 and significantly (P < 0.05) inhibited polymerisation at all concentrations used in comparison to the parallel control. The extract of Mucuna pruriens seed significantly (P < 0.05) increased the solubility of sickle haemoglobin at 50%, 25%, 12.5% and 6.25% concentrations, increased Fe^2+/Fe³⁺ ratio from 1.7 (control) to 12.2 (50% concentration) and reduced osmotic fragility (at 12.5% and 6.25% concentrations) when compared with parallel control. The results indicate the feasibility of the seed extracts as promising agents in the management of sickle cell disease.

镰状细胞病是一组通过基因遗传的疾病，它影响红细胞中的血红蛋白。本实验研究的甲醇种子提取物Buchholzia龟可能的体外抗镰状化的效果，并且还确定的效果刺毛黧豆上的溶解度和镰状细胞血红蛋白的氧结合速率种子提取物。从镰状细胞病患者收集镰状细胞血，随后加入 2% 焦亚硫酸钠以引起更多镰状细胞。将不同浓度的种子提取物（50%、25%、12.5% 和 6.25%）添加到预处理的血液中以进行这些体外测定。结果表明，金缕梅提取物显着（P< 0.05) 在所有浓度下抑制镰状细胞，最高抑制百分比为 73.3 ± 5.8，在所有浓度下逆转镰状红细胞，最高百分比逆转为 83.3 ± 5.8 并且显着 ( P < 0.05) 在所有浓度下抑制聚合，与并行控制。黧豆提取物在50%、25%、12.5%和6.25%浓度下显着提高镰状血红蛋白的溶解度（P < 0.05），提高Fe ^{2+ /} Fe ³⁺比值从 1.7（对照）到 12.2（50% 浓度），与平行对照相比，渗透脆性降低（12.5% 和 6.25% 浓度）。结果表明种子提取物作为治疗镰状细胞病的有希望的药物的可行性。