Significant yield losses are caused by pod shattering during the harvest of domesticated crop plants. The phenotype evaluation of pod shattering requires fully grown plants, and it is a labor-intensive and time-consuming procedure. The purpose of this study was to test the applicability of previously identified KSS-SNP5 SNP marker on chromosome 16 in the marker assisted selection (MAS) system for pod shattering in soybean breeding programs. For this research, TaqMan SNP assay was successfully developed to detect the A/G allele of the KSS-SNP5 marker in the breeding materials, including two breeding stages, F2:3 populations, and yield trial stage breeding lines. The MAS prediction accuracy was calculated by comparing the genotyping data from the TaqMan assay with the phenotype data from the dry-oven method. The total MAS prediction accuracy was 92.5% and 96.2% in two F2:3 populations and breeding lines, respectively and 85% and 96% accuracy were shown in severe conditions. In this study, we confirmed that the KSS-SNP5 TaqMan probe tightly linked to pod shattering is effective to select a resistant line. Based on these results, we propose that the marker tightly linked to pod shattering can be of valuable use in soybean breeding program for the development of pod shattering-resistant cultivars.

中文翻译：

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大豆抗裂果育种计划中标记辅助选择的验证

在驯化作物收获期间，豆荚破碎会导致显着的产量损失。荚果破碎的表型评估需要完全生长的植物，这是一个劳动密集型和耗时的过程。本研究的目的是测试先前在 16 号染色体上鉴定的 KSS-SNP5 SNP 标记在大豆育种计划中用于脱荚的标记辅助选择 (MAS) 系统中的适用性。在本研究中，成功​​开发了TaqMan SNP检测方法，用于检测育种材料中KSS-SNP5标记的A/G等位基因，包括两个育种阶段、F2:3种群和试产阶段育种品系。MAS 预测准确度是通过比较来自 TaqMan 分析的基因分型数据与来自干烤箱方法的表型数据来计算的。MAS 的总预测精度为 92。在两个 F2:3 种群和育种系中分别为 5% 和 96.2%，在恶劣条件下分别显示了 85% 和 96% 的准确率。在本研究中，我们证实与豆荚破碎紧密相关的 KSS-SNP5 TaqMan 探针可有效选择抗性品系。基于这些结果，我们提出与豆荚脱落紧密相关的标记可以在大豆育种计划中用于开发豆荚脱落抗性品种的有价值的用途。