Biodecolorization by microorganisms is a potential treatment technique because they seem to be environmentally safe. In the present study, the decolorization and detoxification of cotton blue, crystal violet, malachite green and methyl violet by endophytic fungi were investigated. Preliminary screening result indicated that SWUSI4, identified as Bjerkandera adusta, demonstrated the best decolorization for the four TPM dyes within 14 days. Furthermore, optimization result demonstrated the decolorization rate could reach above 90% at 24 h by live cells of isolate SWUSI4 when 4 g biomass was added into 100-mL dyes solution with the concentration 50 mg/L and shaking (150 rpm) conditions. Moreover, decolorization mechanism analysis shows that the decolorization was caused by the isolate SWUSI4 that mainly includes both absorption of biomass and/or degradation of enzymes. Biosorption of dyes was attributed to binding to hydroxyl, amino, phosphoryl alkane, and ester–lipids groups based on Fourier transform infrared (FTIR) analyses. The biodegradation potential of SWUSI4 was further suggested by the change of peaks in the ultraviolet–visible (UV–vis) spectra and detection of manganese peroxidase and lignin peroxidase activities. Finally, the phytotoxicity test confirmed that the toxicity of TPM dyes after treatment with SWUSI4 was significantly lower than that before treatment. These results indicate that an endophytic SWUSI4 could be used as a potential TPM dyes adsorption and degradation agent, thus facilitating the study of the plant–endophyte symbiosis in the bioremediation processes.

微生物对生物进行脱色是一种潜在的处理技术，因为它们似乎对环境安全。在本研究中，研究了内生真菌对棉蓝，结晶紫，孔雀石绿和甲基紫的脱色和解毒作用。初步筛选结果表明，鉴定为Bjerkandera adusta的SWUSI4在14天内显示了四种TPM染料的最佳脱色效果。此外，优化结果表明，当将4 g生物质加入浓度为50 mg / L的100 mL染料溶液中并摇动（150 rpm）的条件下，分离的SWUSI4的活细胞在24 h时脱色率可达到90％以上。此外，脱色机理分析表明，脱色是由分离物SWUSI4引起的，其主要包括生物质的吸收和/或酶的降解。根据傅立叶变换红外光谱（FTIR）分析，染料的生物吸附作用归因于与羟基，氨基，磷酰基烷烃和酯-脂质基团的结合。紫外可见光谱的变化以及检测锰过氧化物酶和木质素过氧化物酶的活性进一步表明了SWUSI4的生物降解潜力。最后，植物毒性试验证实，用SWUSI4处理后TPM染料的毒性明显低于处理前。这些结果表明，内生的SWUSI4可用作潜在的TPM染料吸附和降解剂，从而促进了生物修复过程中植物与内生菌共生的研究。