Five deep eutectic solvents (DES) were screened for their effectiveness in improving biosugar yield in Bambara groundnut haulm (BGH) for bioethanol production. Choline chloride-lactic acid (ChCl-LA) treatment provided the most promising result and was further optimised by investigating the effect of temperature and time on cellulose loss and enzymatic sugar yield. ChCl-LA pretreatment at 100 °C for 1 h was observed to be the best condition for hemicellulose (54.5%) and lignin (60.7%) removal along with optimum sugar recovery of 94.8%. The resulting hydrolysate was concentrated and fermented for 72 h with Saccharomyces cerevisiae BY4743, and a maximum ethanol concentration of 11.57 g/L was achieved with an ethanol yield of 0.38 g/g sugar and productivity of 0.19 g/L/h. From the results obtained, it is evident that deep eutectic solvents are effective for breaking the recalcitrance in BGH due to their high delignification capacity. Furthermore, a recovery of the amorphous glucose and xylose in the BGH prior to DES pretreatment is vital to prevent sugar loss, thereby improving the biosugar yield for bioethanol production.

筛选了五种深共熔溶剂（DES），它们可有效改善Bambara花生茎（BGH）中的生物糖产量，以生产生物乙醇。胆碱氯化物-乳酸（ChCl-LA）处理提供了最有希望的结果，并且通过研究温度和时间对纤维素损失和酶促糖产量的影响进行了进一步优化。观察到在100°C下进行ChCl-LA预处理1 h是去除半纤维素（54.5％）和木质素（60.7％）的最佳条件，最佳糖回收率为94.8％。将得到的水解产物浓缩，并用酿酒酵母发酵72小时。BY4743，最大乙醇浓度为11.57 g / L，乙醇产量为0.38 g / g糖，生产率为0.19 g / L / h。从获得的结果可以看出，深共晶溶剂由于其高的脱木质素能力而有效地打破了BGH中的顽固性。此外，在DES预处理之前在BGH中回收无定形葡萄糖和木糖对于防止糖损失是至关重要的，从而提高用于生物乙醇生产的生物糖产量。