Ethanol was produced by separate hydrolysis and fermentation using Azolla filiculoides as a biomass. Thermal acid hydrolysis and enzymatic saccharification were used as pretreatment methods to produce monosaccharides from Azolla. The optimal content for thermal acid hydrolysis of 14% (w/v) Azolla weed slurry produced 16.7-g/L monosaccharides by using 200 mM H₂SO₄ at 121 °C for 60 min. Enzymatic saccharification using 16 U/mL Viscozyme produced 61.6 g/L monosaccharide at 48 h. Ethanol productions with ethanol yield coefficients from Azolla weed hydrolysate using Kluyveromyces marxianus, Candida lusitaniae Saccharomyces cerevisiae, and Pichia stipitis were 26.8 g/L (Y_EtOH = 0.43), 23.2 g/L (Y_EtOH = 0.37), 18.2 g/L (Y_EtOH = 0.29), and 13.7 g/L (Y_EtOH = 0.22), respectively. Saccharomyces cerevisiae produces the lowest yield as it utilized only glucose. Bioethanol from Azolla weed hydrolysate can be successfully produced by using Kluyveromyces marxianus because it consumed the mixture of glucose and xylose completely within 60 h.

使用Azolla filiculoides作为生物质，通过单独的水解和发酵来生产乙醇。热酸水解和酶促糖化被用作从Azolla生产单糖的预处理方法。通过在121°C下使用200 mM H ₂ SO ₄进行60分钟的热酸水解，最佳含量为14％（w / v）的Azolla杂草浆液产生16.7-g / L单糖。使用16 U / mL粘菌酶的酶促糖化作用在48 h产生61.6 g / L单糖。与来自满江红乙醇产量系数乙醇生产剔除水解物使用马克斯克鲁维酵母，假丝酵母葡萄牙念珠菌酿酒酵母，和树干毕赤酵母是26.8克/ L（Y _EtOH中 =  0.43），23.2 g / L（Y _EtOH  =  0.37），18.2 g / L（Y _EtOH  =  0.29）和13.7 g / L（Y _EtOH  =  0.22）。酿酒酵母仅使用葡萄糖，因此产量最低。通过使用马克斯克鲁维酵母可以成功地生产来自Azolla杂草水解产物的生物乙醇，因为它在60小时内完全消耗了葡萄糖和木糖的混合物。