Immobilization of enzyme based on combination of adsorption and cellulose derivative membrane coating was established in this work for the first time. Laccase, a commonly used enzyme in varied fields, was chosen as the model enzyme to demonstrate this method. After investigating operational conditions, the optimal process was obtained as follows: diatomite or HPD-417 as the adsorption carrier, 0.5% (w/v) methylcellulose (40,000~50,000) acetone solution as the coating solution, 0.75% (w/v) polyethylene glycol or maltose as the protective agent, and drying at 4 °C for 9 h. Under the optimal conditions, the residual activities of diatomite and HPD-417 immobilized laccase reached 99.33% and 94.15%, respectively. The study on properties showed that the immobilized laccases held high pH tolerance and thermal stability. The immobilized laccases were further applied to the indigo decolorization and 2, 4-dichlorophenol degradation. They showed high catalytic efficiency and could be reused for several batches. On the whole, the immobilization method developed in this work can effectively avoid the inactivation of laccase during immobilization and improve the stability of immobilized laccase. The laccase immobilized by this method shows obvious potential for environmental governance.

这项工作首次建立了基于吸附和纤维素衍生物膜包衣的酶固定化方法。漆酶是在各种领域中常用的酶，被选作模型酶以证明该方法。在研究了操作条件后，获得了最佳工艺：硅藻土或HPD-417作为吸附载体，0.5％（w / v）甲基纤维素（40,000〜50,000）丙酮溶液作为包衣溶液，0.75％（w / v）聚乙二醇或麦芽糖作为保护剂，并在4°C下干燥9小时。在最佳条件下，硅藻土和HPD-417固定漆酶的残留活性分别达到99.33％和94.15％。性能研究表明，固定化漆酶具有较高的pH耐受性和热稳定性。将固定的漆酶进一步用于靛蓝脱色和2，4-二氯苯酚的降解。它们显示出高催化效率，可以重复使用几批。总体而言，本发明开发的固定化方法可以有效避免固定化过程中漆酶的失活，提高了固定化漆酶的稳定性。用这种方法固定的漆酶具有明显的环境治理潜力。