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Real-time fluorescence sensing of single photoactive proteins using silver nanowires
Methods and Applications in Fluorescence ( IF 2.4 ) Pub Date : 2020-10-05 , DOI: 10.1088/2050-6120/aba7cb
Karolina Sulowska 1 , Kamil Wiwatowski 1 , Maciej Ćwierzona 1 , Joanna Niedziółka-Jönsson 2 , Sebastian Maćkowski 1, 3
Affiliation  

We demonstrate that single functionalized silver nanowires form a geometric platform suitable for efficient real-time detection of single photoactive proteins. By collecting series of images using wide-field fluorescence microscopy, events of single protein attachment can be distinguished with the signal to noise ratio further improved by fluorescence enhancement due to plasmon excitations in the nanowires. The enhancement is evidenced by strong shortening of the fluorescence decay of single photoactive proteins conjugated to the silver nanowires.

中文翻译:

使用银纳米线对单个光活性蛋白进行实时荧光传感

我们证明了单功能化银纳米线形成了一个几何平台,适用于高效实时检测单个光活性蛋白。通过使用宽视场荧光显微镜收集一系列图像,可以区分单个蛋白质附着的事件,并且由于纳米线中的等离子体激发而通过荧光增强进一步提高了信噪比。与银纳米线缀合的单个光活性蛋白的荧光衰减的强烈缩短证明了这种增强。
更新日期:2020-10-06
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