We formed the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM) in 2018 as a cryopreserved fungal collection consolidating the University of Maine Culture Collection (UMCC, or JEL), the University of Alabama Chytrid Culture Collection (UACCC), and additional zoosporic eufungal accessions. The CZEUM is established as a community resource containing 1045 cryopreserved cultures of Chytridiomycota, Monoblepharidomycota, and Blastocladiomycota, with 52 cultures being ex-type strains. We molecularly characterized 431 cultures by amplifying the majority of the rDNA operon in a single reaction, yielding an average fragment length of 4739 bp. We sequenced multiplexed samples with an Oxford Nanopore Technology MinION device and software, and demonstrate the method is accurate by producing sequences identical to published Sanger sequences. With these data, we generated a phylogeny of 882 zoosporic eufungi strains to produce the most comprehensive phylogeny of these taxa to date. The CZEUM is thus largely characterized by molecular data, which can guide instructors and researchers on future studies of these organisms. Cultures from the CZEUM can be purchased through an online portal.

中文翻译：

---

密歇根大学（CZEUM）的游动孢子菌Eufungi馆藏：引入了带条形码的壶菌属和Blastocladiomycota培养物的新存储库

我们于2018年在密歇根大学（CZEUM）成立了动物孢子菌Eufungi菌收藏品，作为冷冻保存的真菌收藏品，巩固了缅因州大学文化收藏品（UMCC或JEL），阿拉巴马大学的柠檬酸培养物收藏品（UACCC）和其他动物孢子虫收藏品真菌加入。建立了CZEUM作为社区资源，其中包含1045种冷冻保存的斜纹菌，单斜纹菌和拟锁霉菌培养物，其中52种培养物是ex型菌株。通过在单个反应中扩增大部分rDNA操纵子，我们对431种培养物进行了分子鉴定，平均片段长度为4739 bp。我们使用牛津纳米孔技术MinION设备和软件对多重样品进行了测序，并通过产生与已发表的Sanger序列相同的序列证明了该方法的准确性。利用这些数据，我们生成了882个孢子虫eufungi菌株的系统发育史，从而产生了迄今为止这些类群中最全面的系统发育史。因此，CZEUM的主要特征是分子数据，它可以指导教师和研究人员对这些生物的进一步研究。可以通过在线门户购买CZEUM的文化。