Optimization of bacterial bioaugmentation for groundwater Mn removal using a waste-based culture medium and lyophilization,Environmental Science: Water Research & Technology

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Optimization of bacterial bioaugmentation for groundwater Mn removal using a waste-based culture medium and lyophilization
Environmental Science: Water Research & Technology ( IF 3.5 ) Pub Date : 2020-10-06 , DOI: 10.1039/d0ew00777c
Lucila Ciancio Casalini _{1,

2,

3,

4,

5} , Micaela Vidoz _{1,

2,

3,

4,

5} , Ainelén Piazza _{1,

2,

3,

4,

5} , Cintia Labanca _{4,

5,

6,

7} , Virginia A. Pacini _{4,

5,

6,

7} , Jorgelina Ottado _{1,

2,

3,

4,

5} , Natalia Gottig _{1,

2,

3,

4,

5}

Affiliation

Biological sand filtration systems are widely used for groundwater Mn removal. Bioaugmentation of sand filters through inoculation with Mn(II)-oxidizing bacteria (MOB) have contributed to the optimization of this biofiltration. However, a challenging aspect of this bioaugmentation process on a large scale is keeping fresh MOB cultures viable and transporting large culture volumes to groundwater treatment plants. In this work, powdered MOB inoculums were prepared by vacuum lyophilization. Bacterial lyophilization was performed in different growth conditions and the best performance was observed in biofilms covered with biogenic Mn oxides. On the other hand, a culture medium to produce the inoculum was developed using crude glycerol waste. Inoculums grown and lyophilized in this glycerol medium were able to be immobilized onto sand filters and to enhance the performance of groundwater Mn removal, reaching the optimal removal efficiency faster than fresh MOB cultures. These results generate new tools to simultaneously, re-use crude glycerol waste and improve large scale bioaugmentation approaches for groundwater Mn removal.

中文翻译：

使用废物培养基和冻干法优化细菌生物强化以去除地下水中的Mn

生物砂滤系统广泛用于去除地下水中的锰。Mn（II）接种对沙滤池的生物强化作用氧化细菌（MOB）有助于优化此生物过滤。但是，这种生物强化过程的一个挑战性方面是保持新鲜的MOB培养物的可行性，并将大量的培养物运输到地下水处理厂。在这项工作中，通过真空冷冻干燥制备了粉末状的MOB接种物。细菌冻干是在不同的生长条件下进行的，在覆盖有生物锰氧化物的生物膜中观察到了最佳性能。另一方面，使用粗制甘油废料开发了产生接种物的培养基。在这种甘油培养基中生长并冻干的接种物能够固定在砂滤器上，并能增强去除地下水中Mn的性能，比新鲜的MOB培养物更快地达到最佳去除效率。

更新日期：2020-11-03

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