当前位置：
X-MOL 学术
›
Chem. Sci.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
In situ visualization of peroxisomal viscosity in the liver of mice with non-alcoholic fatty liver disease by near-infrared fluorescence and photoacoustic imaging
Chemical Science ( IF 7.6 ) Pub Date : 2020-10-06 , DOI: 10.1039/d0sc02922j Yongqing Zhou 1 , Ping Li 1 , Xin Wang 1 , Chuanchen Wu 1 , Nannan Fan 1 , Xiaoning Liu 1 , Lijie Wu 1 , Wei Zhang 1 , Wen Zhang 1 , Zhenzhen Liu 1 , Bo Tang 1
Chemical Science ( IF 7.6 ) Pub Date : 2020-10-06 , DOI: 10.1039/d0sc02922j Yongqing Zhou 1 , Ping Li 1 , Xin Wang 1 , Chuanchen Wu 1 , Nannan Fan 1 , Xiaoning Liu 1 , Lijie Wu 1 , Wei Zhang 1 , Wen Zhang 1 , Zhenzhen Liu 1 , Bo Tang 1
Affiliation
|
Non-alcoholic fatty liver disease (NAFLD) can gradually develop into hepatic failure, and early diagnosis is crucial to improve treatment efficiency. The occurrence of NAFLD is closely related to lipid metabolism. Peroxisomes act as the first and main site for lipid metabolism in the hepatocytes, so abnormal lipid metabolism might directly affect peroxisomal viscosity. Herein, we developed a new near-infrared fluorescence (NIRF) and photoacoustic (PA) imaging probe (PV-1) for the real-time visualization of peroxisomal viscosity in vivo. This PV-1 encompasses the malononitrile group as the rotor, which emits strong NIRF (at 705 nm) and PA (at 680 nm) signals when rotation is hindered as viscosity increases. Through dual-mode imaging, we discovered distinctly higher viscosity in the liver of NAFLD mice for the first time. We further found the remarkable amelioration of NAFLD upon treatment with N-acetylcysteine (NAC). Therefore, we anticipate that the PV-1 imaging method is promising for the early diagnosis and prognostic evaluation of NAFLD.
中文翻译：
通过近红外荧光和光声成像原位可视化非酒精性脂肪肝小鼠肝脏中的过氧化物酶体粘度
非酒精性脂肪性肝病（NAFLD）可逐渐发展为肝衰竭，早期诊断对于提高治疗效率至关重要。 NAFLD的发生与脂质代谢密切相关。过氧化物酶体是肝细胞脂质代谢的首要场所和主要场所，脂质代谢异常可能直接影响过氧化物酶体粘度。在此，我们开发了一种新的近红外荧光（NIRF）和光声（PA）成像探针（PV-1），用于体内过氧化物酶体粘度的实时可视化。该 PV-1 包含丙二腈基团作为转子，当旋转因粘度增加而受到阻碍时，会发出强烈的 NIRF（705 nm）和 PA（680 nm）信号。通过双模式成像，我们首次发现 NAFLD 小鼠肝脏的粘度明显升高。我们进一步发现N-乙酰半胱氨酸 (NAC) 治疗后 NAFLD 得到显着改善。因此，我们预计PV-1成像方法有望用于NAFLD的早期诊断和预后评估。
更新日期：2020-10-19
中文翻译：
通过近红外荧光和光声成像原位可视化非酒精性脂肪肝小鼠肝脏中的过氧化物酶体粘度
非酒精性脂肪性肝病（NAFLD）可逐渐发展为肝衰竭，早期诊断对于提高治疗效率至关重要。 NAFLD的发生与脂质代谢密切相关。过氧化物酶体是肝细胞脂质代谢的首要场所和主要场所，脂质代谢异常可能直接影响过氧化物酶体粘度。在此，我们开发了一种新的近红外荧光（NIRF）和光声（PA）成像探针（PV-1），用于体内过氧化物酶体粘度的实时可视化。该 PV-1 包含丙二腈基团作为转子，当旋转因粘度增加而受到阻碍时，会发出强烈的 NIRF（705 nm）和 PA（680 nm）信号。通过双模式成像，我们首次发现 NAFLD 小鼠肝脏的粘度明显升高。我们进一步发现N-乙酰半胱氨酸 (NAC) 治疗后 NAFLD 得到显着改善。因此，我们预计PV-1成像方法有望用于NAFLD的早期诊断和预后评估。
京公网安备 11010802027423号