Background: Limbal stem cell deficiency (LSCD) is a refractory ocular surface disorder characterized by progressive corneal epithelial degeneration, conjunctivalization, and neovascularization, potentially leading to blindness. There are currently no effective therapeutic options for patients experiencing routine symptomatic treatment failure. Transplantation of amniotic membrane (AM) with adherent stem cells (but not bare AM transplantation alone) has shown promise in preclinical studies for ocular surface restoration. A major limitation, however, is finding a reliable stem cell source. Stem cells can be isolated from the peripheral blood mononuclear cell (PBMC) population, and these PBMC-derived stem cells have numerous advantages over allogeneic and other autologous stem cell types for therapeutic application, including relative ease of acquisition, nonimmunogenicity, and the absence of ethical issues associated with embryonic stem cells.

中文翻译：

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在角膜缘干细胞缺乏兔中使用抗血管内皮生长因子移植外周血单核细胞-羊膜移植后的角膜恢复

背景：边缘干细胞缺陷 (LSCD) 是一种难治性眼表疾病，其特征是进行性角膜上皮变性、结膜形成和新生血管形成，可能导致失明。对于经历常规对症治疗失败的患者，目前没有有效的治疗选择。用贴壁干细胞移植羊膜 (AM)（但不是单独的裸 AM 移植）已在眼表修复的临床前研究中显示出前景。然而，一个主要的限制是寻找可靠的干细胞来源。干细胞可以从外周血单核细胞 (PBMC) 群体中分离出来，这些 PBMC 衍生的干细胞在治疗应用中比同种异体和其他自体干细胞类型具有许多优势，包括相对容易获得，