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Reinforced evidence on partial compatibility between Pinus sylvestris and Pinus mugo and on maternal inheritance of chloroplast DNA in the Pinus mugo × Pinus sylvestris cross
Silvae Genetica ( IF 1.1 ) Pub Date : 2020-01-01 , DOI: 10.2478/sg-2020-0015
Andrej Kormutak 1 , Martin Galgoci 1 , Peter Bolecek 2 , Dusan Gőmőry 3 , Jana Libantova 1
Affiliation  

Abstract The crossability relationship between Scots pine (Pinus sylvestris L.) and mountain dwarf pine (Pinus mugo Turra) was tested under field conditions using an artificial pollination approach. There was partial compatibility between the parental species, as evidenced by the amount of filled seeds in their reciprocal crossings and in control variants from self-pollination, controlled intraspecific outcrossing and open pollination of mother trees. The crossability degree in P. sylvestris × P. mugo was characterised by the index 0.15, and the reciprocal crossing by the index 0.18. Crossability of P. sylvestris and P. mugo with their putative hybrid individuals was much higher; the number of filled seeds was comparable with that of the control variants. The reciprocal crossings of P. sylvestris and P. mugo species were highlighted by the opposite inheritance of their chloroplast DNA (cpDNA). The paternal cpDNA inheritance in P. sylvestris × P. mugo and maternal cpDNA inheritance in P. mugo × P. sylvestris was repeatedly confirmed using the cpDNA trnV-trnH/HinfI marker as well as the newly developed partial cpDNA trnV-trnH/AseI marker. The nature of the latter is described in terms of the nucleotide sequence.

中文翻译:

关于樟子松和麝香松之间部分相容性以及在樟子松×樟子松杂交中叶绿体DNA母系遗传的强化证据

摘要 在田间条件下使用人工授粉方法测试了苏格兰松 (Pinus sylvestris L.) 和山地矮松 (Pinus mugo Turra) 之间的杂交关系。亲本物种之间存在部分相容性,这可以通过其相互杂交中的饱满种子数量以及来自自花授粉、受控种内异交和母树开放授粉的对照变体的数量来证明。P. sylvestris × P. mugo 的可交性程度以指数 0.15 为特征,互交指数为 0.18。P. sylvestris 和 P. mugo 与其假定的杂种个体的杂交性要高得多;饱满种子的数量与对照变体的数量相当。P. sylvestris 和 P. mugo 物种因其叶绿体 DNA (cpDNA) 的相反遗传而突出。使用 cpDNA trnV-trnH/HinfI 标记以及新开发的部分 cpDNA trnV-trnH/AseI 标记反复确认了 P. sylvestris × P. mugo 中的父本 cpDNA 遗传和 P. mugo × P. sylvestris 中的母本 cpDNA 遗传. 后者的性质根据核苷酸序列进行描述。
更新日期:2020-01-01
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