Abstract

For treating tympanic membrane (TM) perforation, a scaffold is helpful for TM repair in myringoplasty. In this work, the green-crosslinked method respectively based on four kinds of polybasic organic acids (maleic acid, tartaric acid, citric acid, and malic acid) has been used to crosslink the composite nanofibrous membranes of chitosan (CS) and poly(vinyl alcohol) (PVA) for preparing the TM scaffold. The crosslinked membranes exhibit nanofibrous morphology, good physical properties, and excellent biocompatibility to promote TM cell proliferation. To summarize, the polybasic organic acids are effective and nontoxic crosslinked agents to prepare composite CS/PVA nanofiber membranes for TM scaffold.

摘要

对于治疗鼓膜 (TM) 穿孔，支架有助于鼓膜成形术中的 TM 修复。在这项工作中，分别基于四种多元有机酸（马来酸、酒石酸、柠檬酸和苹果酸）的绿色交联方法已被用于交联壳聚糖（CS）和聚乙烯（乙烯基）复合纳米纤维膜。酒精) (PVA) 用于制备 TM 支架。交联膜表现出纳米纤维形态、良好的物理特性和优异的生物相容性，可促进 TM 细胞增殖。总之，多元有机酸是制备用于TM支架的复合CS/PVA纳米纤维膜的有效且无毒的交联剂。