Abstract

Matrine has been found to affect cell viability and function. In the present study, we explored the cardioprotective role of matrine in cardiomyocyte damage under hypoxia/reoxygenation. In vitro, cardiomyocyte hypoxia/reoxygenation was used to mimic ischemia/reperfusion injury in the presence of matrine. After exposure to hypoxia/reoxygenation, cardiomyocyte viability was reduced and cell apoptosis was increased; this alteration was inhibited by matrine. At the molecular levels, Sirt3 and AMPK were significantly downregulated by hypoxia/reoxygenation injury whereas matrine administration was able to upregulate Sirt3 and AMPK expression and activity in the presence of hypoxia/reoxygenation. Interestingly, inhibition of Sirt3/AMPK pathway abolished the cardioprotective action of matrine on cardiomyocyte in the presence of hypoxia/reoxygenation injury, resulting into cardiomyocyte viability reduction and cell death augmentation. Altogether, our results demonstrated a novel role played by matrine in regulating cardiomyocyte viability and death in the presence of hypoxia/reoxygenation, with a potential application in the clinical practice for the treatment of patients with myocardial infarction.

摘要

已发现苦参碱影响细胞活力和功能。在本研究中，我们探讨了苦参碱在缺氧/复氧条件下心肌细胞损伤中的心脏保护作用。体外, 心肌细胞缺氧/复氧被用来模拟苦参碱存在下的缺血/再灌注损伤。暴露于缺氧/复氧后，心肌细胞活力降低，细胞凋亡增加；这种改变受到苦参碱的抑制。在分子水平上，Sirt3 和 AMPK 因缺氧/复氧损伤而显着下调，而苦参碱在缺氧/复氧的情况下能够上调 Sirt3 和 AMPK 的表达和活性。有趣的是，在缺氧/复氧损伤存在的情况下，抑制 Sirt3/AMPK 通路消除了苦参碱对心肌细胞的心脏保护作用，导致心肌细胞活力降低和细胞死亡增加。共，