Abstract We have recently applied Raman spectroscopy for biochemical analysis of cell groups treated with 2.5, 5, 7.5 and 10 micromoles per liter of carboplatin and cisplatin, respectively, for apoptosis detection. The continuously decreased intensities of Raman bands assigned to the proteins and deoxyribonucleic acid can be observed as the concentrations of drugs increased, suggesting the cell damage induced by carboplatin and cisplatin. Principal component analysis combined with linear discriminate analysis and support vector machines analysis of the obtained spectral data was used to develop diagnostic algorithms. When cell groups were treated with the same concentration, both principal component analysis combined with linear discriminate analysis and support vector machines analysis executed between control group and groups treated with cisplatin, produce a lower error than those treated with carboplatin, suggesting the more cell damage induced by cisplatin. Among treated groups, an accuracy of 100% is only observed in the group treated with 10 micromoles per liter of cisplatin, demonstrating the apoptosis. Those Statistical analysis results are consistent with the biochemical and Raman spectral analysis. This study indicated that Raman spectroscopy in conjunction with principal component analysis combined with linear discriminate analysis and support vector machines analysis has great potential use in label-free detection of apoptosis in cells and label-free assessment of drug efficacy.

中文翻译：

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卡铂和顺铂对人鼻咽癌细胞的细胞毒作用的无标记拉曼光谱分析

摘要 我们最近应用拉曼光谱对分别用 2.5、5、7.5 和 10 微摩尔/升卡铂和顺铂处理的细胞组进行生化分析，以检测细胞凋亡。随着药物浓度的增加，可以观察到分配给蛋白质和脱氧核糖核酸的拉曼带的强度持续降低，表明卡铂和顺铂诱导了细胞损伤。主成分分析结合线性判别分析和所获得的光谱数据的支持向量机分析被用于开发诊断算法。当细胞组用相同浓度处理时，对照组和顺铂处理组之间的主成分分析结合线性判别分析和支持向量机分析产生的误差均低于卡铂处理组，表明顺铂诱导的细胞损伤更多。在治疗组中，仅在用每升 10 微摩尔顺铂治疗的组中观察到 100% 的准确度，证明了细胞凋亡。这些统计分析结果与生化和拉曼光谱分析一致。该研究表明，拉曼光谱结合主成分分析结合线性判别分析和支持向量机分析，在细胞凋亡的无标记检测和药物疗效的无标记评估方面具有巨大的潜力。产生比用卡铂处理的那些更低的误差，表明顺铂诱导的更多细胞损伤。在治疗组中，仅在用每升 10 微摩尔顺铂治疗的组中观察到 100% 的准确度，证明了细胞凋亡。这些统计分析结果与生化和拉曼光谱分析一致。该研究表明，拉曼光谱结合主成分分析结合线性判别分析和支持向量机分析，在细胞凋亡的无标记检测和药物疗效的无标记评估方面具有巨大的潜力。产生比用卡铂处理的那些更低的误差，表明顺铂诱导的更多细胞损伤。在治疗组中，仅在用每升 10 微摩尔顺铂治疗的组中观察到 100% 的准确度，证明了细胞凋亡。这些统计分析结果与生化和拉曼光谱分析一致。该研究表明，拉曼光谱结合主成分分析结合线性判别分析和支持向量机分析，在细胞凋亡的无标记检测和药物疗效的无标记评估方面具有巨大的潜力。仅在用每升 10 微摩尔顺铂处理的组中观察到 100% 的准确度，证明了细胞凋亡。这些统计分析结果与生化和拉曼光谱分析一致。该研究表明，拉曼光谱结合主成分分析结合线性判别分析和支持向量机分析，在细胞凋亡的无标记检测和药物疗效的无标记评估方面具有巨大的潜力。仅在用每升 10 微摩尔顺铂处理的组中观察到 100% 的准确度，证明了细胞凋亡。这些统计分析结果与生化和拉曼光谱分析一致。该研究表明，拉曼光谱结合主成分分析结合线性判别分析和支持向量机分析，在细胞凋亡的无标记检测和药物疗效的无标记评估方面具有巨大的潜力。