Genomics, transcriptomics, and peptidomics of Spodoptera frugiperda (Lepidoptera, Noctuidae) neuropeptides,Archives of Insect Biochemistry and Physiology

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Genomics, transcriptomics, and peptidomics of Spodoptera frugiperda (Lepidoptera, Noctuidae) neuropeptides
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-10-05 , DOI: 10.1002/arch.21740
Yan Shi ₁ , JiangJie Li ₁ , LinYu Li ₁ , GanLin Lin ₁ , Amir M. Bilal ₁ , Guy Smagghe ₂ , Tong‐Xian Liu ₁

Affiliation

Neuropeptides control many physiological and behavioral processes, and so they are functionally important classes of cell‐to‐cell signaling molecules. Nowadays, the fall armyworm, Spodoptera frugiperda, is one of the most destructive agricultural pests in the world. In this study, we mined the publicly accessible genome assembly data for S. frugiperda, and the transcriptomic and proteomic data of the larval central nervous system (CNS) for putative neuropeptide‐encoding, and subsequently we used these to anticipate a peptidome for this species. In essence, we could identify 57 orthologs of insect neuropeptides, including Allatotropin, CCHamide, Corazonin, pheromone biosynthesis activating neuropeptide, short neuropeptide F, Trissin, and Natalisin. Interesting features for S. frugiperda were the absence of genes coding for CNMamide, Elevein, and the differential evolution of ancestral neuropeptide genes such as adipokinetic corazonin‐related peptide, adipokinetic hormone, Tachykinin, and Natalisin. In conclusion, our study provides the most complete neuropeptide description for the important pest S. frugiperda as a foundation to study the factors regulating insect growth, reproduction, and behavior. Second, we confirm that a comprehensive multi‐omics analysis is necessary for the identification of neuropeptides. Finally, our data provide a reliable reference for other comparative studies in other insects beyond the supermodel insect of Drosophila melanogaster and the finding of potential candidates as selective for pests versus beneficial insects.

中文翻译：

贪夜蛾（Spodoptera frugiperda）（鳞翅目，夜蛾科）神经肽的基因组学，转录组学和肽组学

神经肽控制着许多生理和行为过程，因此它们是细胞间信号分子的重要功能类别。如今，秋天的粘虫Spodoptera frugiperda是世界上最具破坏性的农业害虫之一。在这项研究中，我们提取了可公开获取的弗氏链球菌的基因组装配数据，以及幼虫中枢神经系统（CNS）的转录组和蛋白质组学数据，以推定神经肽编码，随后我们使用它们来预测该物种的肽组。从本质上讲，我们可以鉴定出57种昆虫神经肽的直系同源物，包括同种异体肌钙蛋白，CCH酰胺，可乐松宁，信息素生物合成激活神经肽，短神经肽F，Trissin和Natalisin。有趣的功能草地贪夜蛾是不存在的基因编码CNMamide，Elevein的，和祖神经肽基因如adipokinetic corazonin相关肽，adipokinetic激素，速激肽，Natalisin微分进化。总之，我们的研究为重要的害虫Srug frugiperda提供了最完整的神经肽描述，为研究调节昆虫生长，繁殖和行为的因素奠定了基础。其次，我们确认对神经肽进行鉴定需要进行全面的多组学分析。最后，我们的数据为果蝇超模昆虫以外的其他昆虫的其他比较研究提供了可靠的参考以及发现对害虫和有益昆虫具有选择性的潜在候选物。

更新日期：2020-10-05

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