We investigated spatiotemporal changes in host tissues during foreign body reactions. Silicone tube was subcutaneously embedded into ICR mice, and tissue surrounding silicone (TSS) was observed at 2, 7, 14, 21, 28, 43, and 70 days (D) postsurgery. The thin layer (TL) and loose connective tissues (LCTs) (inside and outside the TSS) developed until D21 and densified afterward. Neutrophils infiltrated the TSS until D14 and formed neutrophil extracellular traps (NETs) in the TL during D7‐21. In the LCTs, mast cell counts increased until D21, and macrophage numbers peaked at D14. Several macrophages showed LYVE‐1 expression, supporting a tissue‐remodeling role. Developmental indices of collagen fibers (CFs) and reticular fibers (RFs) increased during D2‐21. NETs, but not neutrophils, were detected after D28. Mast cell numbers peaked at D43 and were maintained until D70. Myofibroblasts consistently localized to the TL from D14. During D21‐28, the area of connective tissue (CNT), and CFs and RFs decreased and increased, respectively, and both remained constant during D28‐70. The CF density remained constant from D21 and increased at D70. Thus, TSS showed two phases: inflammation and CNT development (D2‐21), and inflammation convergence and CNT stabilization (D28‐70). These results provide insights into foreign body reactions in clinical cases.

中文翻译：

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硅胶异物反应过程中小鼠皮下组织的时空组织学变化

我们调查了异物反应过程中宿主组织的时空变化。硅胶管被皮下嵌入 ICR 小鼠中，并在术后 2、7、14、21、28、43 和 70 天 (D) 观察硅胶周围的组织 (TSS)。薄层 (TL) 和松散结缔组织 (LCT)（TSS 内部和外部）发育至 D21 并随后致密。中性粒细胞浸润 TSS 直到 D14，并在 D7-21 期间在 TL 中形成中性粒细胞胞外陷阱 (NETs)。在 LCT 中，肥大细胞计数增加至 D21，巨噬细胞数量在 D14 达到峰值。几个巨噬细胞表现出 LYVE-1 表达，支持组织重塑作用。胶原纤维 (CFs) 和网状纤维 (RFs) 的发育指数在 D2-21 期间增加。在 D28 后检测到 NET，但未检测到中性粒细胞。肥大细胞数在 D43 达到峰值并维持到 D70。从 D14 开始，肌成纤维细胞始终定位于 TL。在 D21-28 期间，结缔组织 (CNT) 的面积、CFs 和 RFs 分别减少和增加，并且在 D28-70 期间均保持不变。CF 密度从 D21 开始保持不变，并在 D70 增加。因此，TSS 表现出两个阶段：炎症和 CNT 发育 (D2-21)，以及炎症收敛和 CNT 稳定 (D28-70)。这些结果为临床病例中的异物反应提供了见解。TSS 显示两个阶段：炎症和 CNT 发展 (D2-21)，以及炎症收敛和 CNT 稳定 (D28-70)。这些结果为临床病例中的异物反应提供了见解。TSS 显示两个阶段：炎症和 CNT 发展 (D2-21)，以及炎症收敛和 CNT 稳定 (D28-70)。这些结果为临床病例中的异物反应提供了见解。