κ-Carrageenase (EC3.2.1.83) is a class of glycoside hydrolase, which can be used for hydrolysis of κ-carrageenan to κ-carrageenan oligosaccharides. In this study, a bacterium, identified as Pseudoalteromonas sp. ZDY3 isolated from rotten algae, was capable to degrade κ-carrageenan. The κ-carrageenase produced by Pseudoalteromonas sp. ZDY3 was purified to homogeneity and named as CgkZDY3. The accurate molecular mass of CgkZDY3 was determined through LC-HRMS, and a posttranslational removal of C-terminal end of the protein was discovered. CgkZDY3 had strict hydrolysis specificity to κ-carrageenan, the values of K_m and k_cat/K_m of CgkZDY3 were 3.67 mg mL⁻¹ and 53.0 mL mg⁻¹ s⁻¹, respectively. CgkZDY3 was a cold-adapted κ-carrageenase with excellent storage stability of both the temperature below 35 °C and a wide pH range, and was an endo-type κ-carrageenase with high hydrolysis rate, oligosaccharides with different degrees of polymerization can be obtained by controlling the hydrolysis time, and the final products were κ-neocarrabiose and κ-neocarratetraose. These properties are of great significance for production of κ-carrageenan oligosaccharides with different polymerization degrees under process control.

κ-角叉菜胶酶（EC3.2.1.83）是一类糖苷水解酶，可用于将κ-角叉菜胶水解为κ-角叉菜胶寡糖。在这项研究中，一种细菌被鉴定为假单胞菌属。从腐烂的藻类中分离出的ZDY3能够降解κ角叉菜胶。假单胞菌属sp产生的κ-角叉菜胶酶。将ZDY3纯化至均质，并将其命名为CgkZDY3。通过LC-HRMS确定CgkZDY3的准确分子量，并发现翻译后去除了蛋白质的C末端。CgkZDY3对κ-角叉菜胶具有严格的水解特异性，K _m和k _cat / K _m的值CgkZDY3的Mg分别为3.67mg mL ^-1和53.0mL mg ^-1  s ^-1。CgkZDY3是一种低温适应的κ-角叉菜胶酶，在低于35°C的温度和宽pH范围内均具有优异的储存稳定性，并且是一种内切型κ-角叉菜胶酶，具有较高的水解速率，可以得到不同聚合度的寡糖。通过控制水解时间，最终产物为κ-新芥酸和κ-新四糖。这些性质对于在工艺控制下生产不同聚合度的κ-角叉菜胶低聚糖具有重要意义。