Three carbamidocyclophanes, A, F and V, and carbamidocylindrofridin A were isolated from the cultured freshwater cyanobacterium Cylindrospermum stagnale, collected in the Canary Islands. The chemical structures of these compounds were elucidated through NMR, HRMS and ECD spectroscopy. The absolute configuration of carbamidocyclophane A was confirmed using X-ray-diffraction. All compounds showed apoptotic capacity against the SK-MEL-1, SK-MEL-28 and SK-MEL-31 tumour cells. Carbamidocylindrofridin A had the highest anti-tumour potential, with an IC50 of 1 ± 0.3 μM in the SK-MEL-1 cell line.

中文翻译：

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从蓝藻Cylindrospermum stagnale BEA 0605B 中分离出的carbamidocyclophanes 和carbamidocylindrofridin A 的抗肿瘤潜力

从在加那利群岛收集的培养的淡水蓝藻 Cylindrospermum stagnale 中分离出三种 carbamidocyclophanes，A、F 和 V，以及 carbamidocylindrofridin A。通过NMR、HRMS和ECD光谱阐明了这些化合物的化学结构。carbamidocyclophane A 的绝对构型通过 X 射线衍射确认。所有化合物均显示出针对 SK-MEL-1、SK-MEL-28 和 SK-MEL-31 肿瘤细胞的凋亡能力。Carbamidocylindrofridin A 具有最高的抗肿瘤潜力，在 SK-MEL-1 细胞系中的 IC50 为 1 ± 0.3 μM。