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A novel glycoprotein from Streptomyces sp. triggers early responses of plant defense
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.pestbp.2020.104719 Lirong Han 1 , Yubo Sun 1 , Xin Zhou 1 , Xinchang Hao 1 , Meng Wu 1 , Xing Zhang 1 , Juntao Feng 1
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.pestbp.2020.104719 Lirong Han 1 , Yubo Sun 1 , Xin Zhou 1 , Xinchang Hao 1 , Meng Wu 1 , Xing Zhang 1 , Juntao Feng 1
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GP-1, a novel glycoprotein from Streptomyces sp. ZX01 has a plant immunity-inducing effect. GP-1-treated plants exhibited enhanced systemic resistance with a significant reduction in TMV lesions on tobacco leaves, but its antiviral mechanism remains unclear. In this study, early plant defense-related responses, such as Ca2+ influx, callose apposition, oxidative burst, hypersensitive response, programmed cell death, increase in nitric oxide (NO), and stomatal closure, were investigated under GP-1 treatment, and the mechanism of how GP-1 induces viral resistance in Nicotiana benthamiana was studied. Results showed that GP-1 induced [Ca2+]cyt rapidly in tobacco leaves and suspended cells, followed by reactive oxygen species (ROS) and NO elevation. Transcriptome analysis showed significant differences in expression levels between the GP-1-treated N. benthamiana and the control and showed significantly upregulated and enriched pathways including defense and immune reaction. Similar to typical pathogen-associated molecular patterns, GP-1 induced callose deposition and stomatal closure to form defense barriers against pathogen invasion. The expression of defense-related genes further confirmed the above conclusions. By analyzing transcriptome in N. benthamiana and the contents of salicylic acid (SA) and jasmonic acid (JA), GP-1 enhanced viral resistance of tobacco by improving the SA and JA contents, strengthening plant secondary metabolites activities, promoting systemic accumulation of pathogenesis-related proteins in TMV- inoculated tobacco there by producing antiviral activity.
中文翻译：
来自链霉菌属的一种新型糖蛋白。触发植物防御的早期反应
GP-1，一种来自链霉菌属的新型糖蛋白。 ZX01具有植物免疫诱导作用。 GP-1处理的植物表现出增强的系统抗性，烟叶上的TMV损伤显着减少，但其抗病毒机制仍不清楚。在本研究中，在 GP-1 处理下研究了早期植物防御相关反应，如 Ca2+ 流入、胼胝质并置、氧化爆发、过敏反应、程序性细胞死亡、一氧化氮 (NO) 增加和气孔关闭，研究了GP-1如何诱导本塞姆氏烟草病毒抗性的机制。结果表明，GP-1 快速诱导烟叶和悬浮细胞中的 [Ca2+]cyt，随后导致活性氧 (ROS) 和 NO 升高。转录组分析显示，经 GP-1 处理的本塞姆氏烟草和对照之间的表达水平存在显着差异，并显示出包括防御和免疫反应在内的途径显着上调和富集。与典型的病原体相关分子模式类似，GP-1 诱导胼胝质沉积和气孔关闭，形成防御病原体入侵的防御屏障。防御相关基因的表达进一步证实了上述结论。通过对本塞姆氏烟转录组及水杨酸（SA）和茉莉酸（JA）含量的分析，GP-1通过提高SA和JA含量，增强植物次生代谢产物活性，促进病机系统积累，增强烟草的病毒抗性。接种TMV的烟草中的相关蛋白通过产生抗病毒活性而存在。
更新日期：2021-01-01
中文翻译：
来自链霉菌属的一种新型糖蛋白。触发植物防御的早期反应
GP-1，一种来自链霉菌属的新型糖蛋白。 ZX01具有植物免疫诱导作用。 GP-1处理的植物表现出增强的系统抗性，烟叶上的TMV损伤显着减少，但其抗病毒机制仍不清楚。在本研究中，在 GP-1 处理下研究了早期植物防御相关反应，如 Ca2+ 流入、胼胝质并置、氧化爆发、过敏反应、程序性细胞死亡、一氧化氮 (NO) 增加和气孔关闭，研究了GP-1如何诱导本塞姆氏烟草病毒抗性的机制。结果表明，GP-1 快速诱导烟叶和悬浮细胞中的 [Ca2+]cyt，随后导致活性氧 (ROS) 和 NO 升高。转录组分析显示，经 GP-1 处理的本塞姆氏烟草和对照之间的表达水平存在显着差异，并显示出包括防御和免疫反应在内的途径显着上调和富集。与典型的病原体相关分子模式类似，GP-1 诱导胼胝质沉积和气孔关闭，形成防御病原体入侵的防御屏障。防御相关基因的表达进一步证实了上述结论。通过对本塞姆氏烟转录组及水杨酸（SA）和茉莉酸（JA）含量的分析，GP-1通过提高SA和JA含量，增强植物次生代谢产物活性，促进病机系统积累，增强烟草的病毒抗性。接种TMV的烟草中的相关蛋白通过产生抗病毒活性而存在。
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