Carbapenemase Inactivation Method (CIM) is a test to detect presence of the carbapenemase in Gram-negative bacteria. Determination of the carbapenemase production by inactivation of meropenem requires that a zone of control E. coli inhibition be measured approximately 6–24 h after plating. We have modified the CIM test by developing a rapid method which instead measures the growth of E. coli indicator strain ATCC 25922 using real-time PCR, referred to as a nucleic acid testing CIM (natCIM). Our natCIM, therefore reduces the detecting time from 6 to 24 h to approximately 4 h.

中文翻译：

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通过实时 PCR 减少碳青霉烯酶灭活方法 (CIM) 测定时间

碳青霉烯酶灭活方法 (CIM) 是一种检测革兰氏阴性菌中是否存在碳青霉烯酶的测试。通过灭活美罗培南来测定碳青霉烯酶的产生需要在铺板后约 6-24 小时测量大肠杆菌抑制控制区。我们通过开发一种快速方法对 CIM 测试进行了修改，该方法使用实时 PCR 来测量大肠杆菌指示菌株 ATCC 25922 的生长，称为核酸测试 CIM (natCIM)。因此，我们的 natCIM 将检测时间从 6 至 24 小时减少到大约 4 小时。