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Chemically modified mRNA nucleofection of primary human T cells
Journal of Immunological Methods ( IF 1.6 ) Pub Date : 2020-10-06 , DOI: 10.1016/j.jim.2020.112878 Nikolaus Thuille 1 , Tajana Sajinovic 1 , Kerstin Siegmund 1 , Gottfried Baier 1
中文翻译:
原代人 T 细胞的化学修饰 mRNA 核转染
更新日期:2020-11-25
Journal of Immunological Methods ( IF 1.6 ) Pub Date : 2020-10-06 , DOI: 10.1016/j.jim.2020.112878 Nikolaus Thuille 1 , Tajana Sajinovic 1 , Kerstin Siegmund 1 , Gottfried Baier 1
Affiliation
Here we show that an approach of in-vitro transcribed mRNA nucleofection expands the range of transfection of primary human T cells. It represents a reproducible and time-efficient technology, and is thus an ideal tool in basic research involving highly controlled in-vitro experiments with a gene of interest aiming at identifying its biological human T cell function.
中文翻译:
原代人 T 细胞的化学修饰 mRNA 核转染
在这里,我们展示了体外转录 mRNA 核转染的方法扩大了原代人类 T 细胞的转染范围。它代表了一种可重复且省时的技术,因此是基础研究中的理想工具,涉及高度受控的体外实验,目的是确定目标基因的生物人类 T 细胞功能。