Here we show that an approach of in-vitro transcribed mRNA nucleofection expands the range of transfection of primary human T cells. It represents a reproducible and time-efficient technology, and is thus an ideal tool in basic research involving highly controlled in-vitro experiments with a gene of interest aiming at identifying its biological human T cell function.

中文翻译：

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原代人 T 细胞的化学修饰 mRNA 核转染

在这里，我们展示了体外转录 mRNA 核转染的方法扩大了原代人类 T 细胞的转染范围。它代表了一种可重复且省时的技术，因此是基础研究中的理想工具，涉及高度受控的体外实验，目的是确定目标基因的生物人类 T 细胞功能。